Search

Background: Anopheles sinensis is one of the major malaria vectors in China and other southeast Asian countries, including Vietnam, Cambodia, Thailand. Vector control is considered to be the critical measure for malaria control, while the increasing prevalence of insecticide resistance caused by long-term use of insecticides, especially pyrethroids, is threatening the successful control of An. sinensis. In order to understand the underlying resistance mechanisms involved and molecular basis, the principal malaria vector, An. sinensis from Jiangsu and Anhui provinces, Southeast China, was investigated. Methods: The adult Anopheles mosquitoes were sampled from multiple sites across Jiangsu and Anhui provinces, and sufficient mosquitoes collected from eleven sites for insecticide susceptibility bioassays. The DIIS4–DIIS6 region of the para-type sodium channel gene was amplified and sequenced, then multiple PCR and Taqman assays were used to assess the frequencies of kdr mutations at the target gene. Results: In the present study, most of the adult An. sinensis populations were pyrethroids resistant, which indicated the presence of kdr resistance mutations in the para-type sodium channel gene. Sequence analyses demonstrated the kdr mutation existed at codon 1014 in Jiangsu and Anhui provinces. In adult An. sinensis, three mutant types (TTT L1014F, TTC L1014F, and TGT L1014C) of kdr alleles were detected, while no wild type (TTG L1014) was observed. The TTC L1014F mutation was first reported in Anhui province. Conclusions: The highly polymorphic kdr alleles were observed in all the adult An. sinensis populations, which suggested that in-depth studies are required for carrying on insecticide resistance monitoring and specific resistance mechanisms studying into establish effective long-term malaria vector control program in eastern China. [ABSTRACT FROM AUTHOR]

This study investigated the wintertime vertical distributions and source areas of aerosols, NO 2 , and HCHO in a coastal city of Dongying from December 2020 to March 2021, using ground-based multi-axis differential optical absorption spectroscopy (MAX-DOAS) and a potential source contribution function (PSCF) model, respectively. Moreover, the chemical production sensitivity of O 3 at different height layers was analyzed using HCHO/NO 2 ratios. The results revealed that the wintertime averaged highest concentrations of aerosol (1.25 km−1), NO 2 (14.81 ppb), and HCHO (2.32 ppb) were mainly distributed at the surface layer, 100–200 m layer, and 200–300 m layer, respectively. Regarding the diurnal cycles, high concentrations of aerosol (>1.4 km−1) and NO 2 (>16.0 ppb) usually appeared in the early morning and late afternoon, while high concentrations of HCHO (>2.5 ppb) usually occurred during 12:00–15:00. The PSCF model revealed that the wintertime aerosol mainly originated from Shandong, northern Jiangsu, Korea, and the northwestern Mongolian Plateau. Below 200 m, NO 2 was mainly from western Shandong, whereas above 600 m, it was mainly from northern Shandong and the Beijing-Tianjin-Hebei (BTH) region. The corresponding sources for HCHO were central and southern Shandong (below 200 m) and northern Shandong, northern Jiangsu, and southeastern BTH (above 600 m). In addition, the chemical production sensitivity of O 3 below 100 m was observed only in the VOC-limited regime. The percentages of O 3 production under the NO x -limited, NO x -VOC-limited, and VOC-limited regimes were 10.75% (31.18%), 4.30% (19.35%), and 84.95% (49.47%) at the 500–600 m (900–1000 m) layer. This study has guiding significance for the coordinated control of PM 2.5 and O 3 , and can assist in the implementation of regional joint prevention and control strategies for air pollutants. • The averaged wintertime NO 2 and HCHO profiles all exhibited a Gaussian shape. • The vertical potential sources of aerosol, NO 2 and HCHO were analyzed using PSCF. • O 3 chemical production sensitivity under 100 m was only VOC-limited. • The percentages of VOC-limited O 3 production at 500–600 were 84.95%. [ABSTRACT FROM AUTHOR]