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This study aims to evaluate the effect of employing infographics in higher education, specifically with regard to students’ interactions and their perception of the meaning of transferred information. This evaluation was presented through lectures at Applied Sciences Private University in Jordan and two other selected colleges (one scientific and the other humanistic) to compare the two variables applied in this study. To achieve this object infographic materials were developed by researcher and guided by (ASSURE) instructional model to present the lectures to the two experimental groups by infographic method through two selected courses from Information Technology College and Business College. Meanwhile, two control groups were taking the same courses in deferent class by conventional method. In addition, the total of participants was (138 students) and that includes all students of the experimental and control groups. Furthermore, a post-test questionnaire was involved to experimental groups to measure their interaction and perception after presenting lectures by infographic materials. The findings of this study show that infographics have a positive and strong impact on students’ interaction and perception. Furthermore, a comparison shows that the students of the scientific and humanistic colleges had different effect of interaction. However, infographics have a similar effect on perception in both colleges. [ABSTRACT FROM AUTHOR]