1. Molecular detection of Fowl adenovirus from broiler chicken in Kerala.
- Author
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Ayana, B. S., Sankar, S., Priya, P. M., Nair, S. R., and Krishna, B. D.
- Subjects
ADENOVIRUSES ,BROILER chickens ,BROILER chicken diseases ,HEPATITIS - Abstract
Fowl adenovirus affects birds, mainly chicken, duck, geese, turkey, and pheasant. Fowl adenovirus comprises of 5 species and 12 serotypes, which causes a multitude of disease syndromes. The most economically significant diseases caused by Fowl adenovirus include inclusion body hepatitis and hepatitis-hydropericardium syndrome. In the present study, a total of 56 post-mortem samples were collected from broiler birds brought to the Department of Veterinary Microbiology, Department of Veterinary Pathology and some organised farms in Kerala. Postmortem samples of liver, heart, lungs, kidney, bursa of Fabricius and spleen were collected for molecular detection. DNA was extracted from the pooled tissue samples using phenol chloroform method and subjected to polymerase chain reaction, employing primers targeting the hexon gene. Seven out of 56 samples turned out to be positive in PCR (12 per cent). The study revealed the increasing prevalence of Fowl adenovirus among the broiler population of Kerala. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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