1. Vigna angularis inhibits IL-6-induced cellular signalling and ameliorates collagen-induced arthritis.
- Author
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Oh, Hyun-Mee, Lee, Seung Woong, Yun, Bo Ra, Hwang, Byung Soon, Kim, So Nam, Park, Chan Sun, Jeoung, See-Hwa, Kim, Hyun-Kyu, Lee, Woo Song, and Rho, Mun-Chual
- Subjects
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ARTHRITIS prevention , *ACADEMIC medical centers , *ALTERNATIVE medicine , *ANIMAL experimentation , *ARTHRITIS , *ENZYME-linked immunosorbent assay , *INTERLEUKINS , *MICE , *RESEARCH funding , *T-test (Statistics) , *WESTERN immunoblotting , *DATA analysis software , *CHEMICAL inhibitors ,THERAPEUTIC use of plant extracts - Abstract
Objectives. The present study was conducted in order to assess whether extracts or isolated compounds from Vigna angularis were able to suppress IL-6 signalling and to show the therapeutic effect on collagen-induced arthritis (CIA) in mice.Methods. The effect of V. angularis on IL-6 signalling was studied by measuring Stat3-dependent luciferase activity, expression of inflammation-related genes, and phosphorylation of Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3) and extracellular signal-regulated kinase (ERK) induced by IL-6. CIA was induced by immunizing with bovine type II collagen. V. angularis extract (VAE) was administrated orally at 50 and 100 mg/kg from day 1 to day 28. Induction of arthritis was evaluated with a visual scoring system and histological analysis.Results. Extracts or two triterpenoid compounds from V. angularis showed potent inhibitory effects on pSTAT3-inducible luciferase activity, STAT3 tyrosine phosphorylation and the expression of inflammation-related genes induced by IL-6. Administration of VAE significantly suppressed the progression of CIA, accompanied by a reduced antibody response to type II collagen and protection from tissue damage in knee joints.Conclusion. Administration of VAE has a therapeutic effect on CIA and this effect is associated with the inhibitory activity on IL-6/STAT3 signalling. These results suggest that extracts or compounds from V. angularis could be a useful treatment for diseases related to IL-6, including RA. [ABSTRACT FROM PUBLISHER]
- Published
- 2014
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