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The number, size and severity of aquatic low-oxygen dead zones are increasing worldwide. Microbial processes in low-oxygen environments have important ecosystem-level consequences, such as denitrification, greenhouse gas production and acidification. To identify key microbial processes occurring in low-oxygen bottom waters of the Chesapeake Bay, we sequenced both 16S rRNA genes and shotgun metagenomic libraries to determine the identity, functional potential and spatiotemporal distribution of microbial populations in the water column. Unsupervised clustering algorithms grouped samples into three clusters using water chemistry or microbial communities, with extensive overlap of cluster composition between methods. Clusters were strongly differentiated by temperature, salinity and oxygen. Sulfur-oxidizing microorganisms were found to be enriched in the low-oxygen bottom water and predictive of hypoxic conditions. Metagenome-assembled genomes demonstrate that some of these sulfur-oxidizing populations are capable of partial denitrification and transcriptionally active in a prior study. These results suggest that microorganisms capable of oxidizing reduced sulfur compounds are a previously unidentified microbial indicator of low oxygen in the Chesapeake Bay and reveal ties between the sulfur, nitrogen and oxygen cycles that could be important to capture when predicting the ecosystem response to remediation efforts or climate change., (© 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.)

This study evaluated the impact that Freddie Gray's death on April, 19, 2015, had on homicides within Baltimore, Prince George's County, and the rest of Maryland. The numbers of these deaths were compared in the 2 years prior and after his death. The average number of homicide per month in Baltimore and Prince George's County, but not the rest of Maryland, showed a statistically significant increase in the 2 years after his death, with a sudden spike in homicides in Baltimore immediately following his death. Spatial density ("heat") maps of homicides showed an increase in clustering of deaths after Freddie Gray's demise in portions of Baltimore, particularly near his arrest site. Hot spot maps showed a massive expansion of statistically significant clusters of homicides in areas of West and East Baltimore after his death. An emerging hot spot analysis revealed consecutive hot spots in large areas of West Baltimore with new hot spots prominent along the southern edge of this area. A review of the history of Baltimore, as well as discussions with Baltimore homicide detectives, revealed that prior socioeconomic and political trends likely primed Baltimore for the protests and violence that occurred after Freddie Gray's death. It is probable that a perceived "overcharging" of officers involved in his death by the State's Attorney resulted in a "pullback" from policing that directly led to increased homicides in Baltimore., (© 2020 American Academy of Forensic Sciences.)

This study examines how therapeutic change in couples is facilitated in the Cultural Context Model based on the model's identified family processes: critical consciousness, empowerment, and accountability. Participants included four female therapists and three couples who sought treatment voluntarily, two heterosexual couples, and one lesbian couple with diverse class and ethnic backgrounds who had spent at least 12 months receiving services at the Institute for Family Services and who were compliant with treatment. Videotaped sessions were analyzed by coding descriptive data using a constant comparative analysis method. An analysis of therapeutic interventions and clients' changes showed that the model works in four realms: conversational, behavioral, ritual, and community building.