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The aim of this study was to quantify and qualify the water for irrigation in the watershed of Rio Formoso Indigenous Land in Tangara da Serra, Mato Grosso, Brazil, in the condition of an important Brazilian agricultural center. Five river basins were delineated with five collection points and sampling in 12 consecutive months, starting in August / 2016. The following 15 variables were evaluated: nitrogen (N), phosphorus (P), temperature (T), turbidity (Tu), total solids (TS), potassium (K+), calcium (Ca2+), magnesium (Mg2+), chlorides (Cl-), bicarbonates (HCO3 -), electrical conductivity (EC), potential of hydrogen (pH), thermotolerant coliforms (T.C.), alkalinity (Alk) and sodium (Na+) and the ratio of sodium absorption (RSA) and hardness (Har GHF) was calculated. The results revealed no risk of salinization, toxicity and severe risk of sodification. The potential of hydrogen is low and the water temperature is above 25°C. The concentration of thermotolerant coliforms at three points damages the cultivation of hardwoods that are consumed raw. The disconformities found indicate that the water use should be done with specialized technical assistance to avoid irreversible damage to the soil and equipments. There is no limitation of water quantity of available flow grantable of 1,884 m? s-?. [ABSTRACT FROM AUTHOR]