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In the present study, Life Cycle Assessment (LCA) methodology was applied to evaluate the energy consumption and environmental burdens associated with the production of protected crops in an agricultural district in the Mediterranean region. In this study, LCA was used as a ‘support tool’, to address local policies for sustainable production and consumption patterns, and to create a ‘knowledge base’ for environmental assessment of an extended agricultural production area. The proposed approach combines organisation-specific tools, such as Environmental Management Systems and Environmental Product Declarations, with the environmental management of the district. Questionnaires were distributed to producers to determine the life cycle of different protected crops (tomatoes, cherry tomatoes, peppers, melons and zucchinis), and obtain information on greenhouse usage (e.g. tunnel vs. pavilion). Ecoprofiles of products in the district were also estimated, to identify supply chain elements with the highest impact in terms of global energy requirements, greenhouse gas emissions, eutrophication, water consumption and waste production. These results of this study enable selection of the ‘best practices’ and ecodesign solutions, to reduce the environmental impact of these products. Finally, sensitivity analysis of key LCA issues was performed, to assess the variability associated with different parameters: vegetable production; water usage; fertiliser and pesticide usage; shared greenhouse use; substitution of plastics coverings; and waste recycling. [Copyright &y& Elsevier]