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Abstract Abundance and qualitative composition of benthic marine litter were investigated in a coastal area of the central Mediterranean Sea. Almost 30 km of video footage, collected by a Remotely Operated Vehicle between 5 and 30 m depth, were analyzed. Litter density ranged from 0 to 0.64 items/m2 with a mean of 0.11 (±0.16) items/m2. General wastes, made up almost entirely of plastic objects, were the dominant sources of debris representing 68% of the overall litter. The remaining 32% consisted of lost or abandoned fishing gears. Synthetic polymers, considering both fishing gears and general waste, represented 73% of total debris items. Our results are comparable with litter amounts reported in other Mediterranean sites at similar depths. Overall, the results are discussed in terms of monitoring strategy, to support the implementation of the Marine Strategy Framework Directive (2008/56/EC) for descriptor 10 and the Mediterranean UN Environment (UNEP/MAP) regional Plan on Marine Litter. Highlights • A total of 56 litter items were observed by ROV on a shallow area of 59,616 m2. • The mean density of marine litter for the whole area was 0.11 items 100 m−2. • General wastes represented 67.86% of the overall density. • 73% of all marine litter items was composed by plastics. • Ropes, disposable crockeries and papers accounted for 50% of the total density. [ABSTRACT FROM AUTHOR]

Marine litter is commonly observed everywhere in the ocean. In this study, we analyzed 17 km of video footage, collected by a Remotely Operated Vehicle (ROV) at depths ranging between 20 and 220 m, during 19 transects performed on the rocky banks of the Straits of Sicily. Recently, the Contracting Parties of the Convention on Biological Diversity (CBD) recognized this site as an Ecologically or Biologically Significant Area (EBSA). The research aim was to quantify the abundance of marine litter and its impact on benthic fauna. Litter density ranged from 0 items/100 m 2 to 14.02 items/100 m 2 with a mean (±standard error) of 2.13 (±0.84) items/100 m 2 . The observed average density was higher (5.2 items/100 m 2 ) at depths >100 m than at shallower depths (<100 m, 0.71 items/100 m 2 ). Lost or abandoned fishing lines contributed to 98.07% of the overall litter density, then representing the dominant source of marine debris. Litter interactions with fauna were frequently observed, with 30% of litter causing “entanglement/coverage” and 15% causing damage to sessile fauna. A total of 16 species showed interaction (entanglement/coverage or damage) with litter items and 12 of these are species of conservation concern according to international directives and agreements (CITES, Berne Convention, Habitat Directive, SPA/BD Protocol, IUCN Red List); we also observed 7 priority habitats of the SPA/BD Protocol. This research will support the implementation of monitoring “Harm” as recommended by the UN Environment/MAP Regional Plan on Marine Litter Management in the Mediterranean, and the Marine Strategy Framework Directive (MSFD). The institution of a SPAMI in the investigated area could represent a good management action for the protection of this hotspot of biodiversity and to achieve a Good Environmental Status (GES) for the marine environment by 2020, under the MSFD. [ABSTRACT FROM AUTHOR]

The aim of the present study was to evaluate the hazard from shipwrecks on communities by a holistic approach taking into account different effects on biological communities. Multibeam and Remotely Operated Vehicles surveys recorded ecological assessment of fish and benthic species on three shipwrecks flooded during the Second World War on Maërl beds habitats in the strait of Sicily. Pollution levels of a wide range of chemicals of ecotoxicological concern were also measured in sediments and in fish species from different trophic levels. Statistical analysis evidenced significant differences among pollutant levels between both sediments and fish collected in shipwreck sites and controls. Concerning fish, significant effects due to the vessel's cargo type and flooding position are recorded. In spite of that, our results underline that shipwrecks are also a hotspots of biodiversity and a habitat for preservation strategies in marine ecosystems that need to be monitored. [ABSTRACT FROM AUTHOR]

The present paper investigates the diet and the food composition ofTrachinotus ovatusin the central Mediterranean Sea (Strait of Messina). Moreover, the first documented data on plastic ingestion byT. ovatusare also reported. Samples ranging between 16.5 and 28.0 cm fork length were collected between May and November 2012 in the Strait of Messina (central Mediterranean Sea) by trolling lines.T. ovatusfed mainly on pelagic crustaceans and fishes, although the contribution of mollusks was also important. The absence of dominant prey indicated a generalist feeding behavior. The plastic debris was found in the stomach content ofT. ovatuswith a high percentage of occurrence (%O = 24.3%). Considering the commercial interest thatT. ovatushas in some small-scale fishery markets, the potential impact of plastics on the trophic web and human consumption should be investigated. [ABSTRACT FROM AUTHOR]

Length-mass relationships and linear regressions are given for otolith size (length and height) and standard length ( LS) of certain mesopelagic fishes (Myctophidae, Paralepididae, Phosichthyidae and Stomiidae) living in the central Mediterranean Sea. The length-mass relationship showed isometric growth in six species, whereas linear regressions of LS and otolith size fit the data well for all species. These equations represent a useful tool for dietary studies on Mediterranean marine predators. [ABSTRACT FROM AUTHOR]

New records of the rare Messina rockfishScorpaenodes arenai(Osteichthyes: Scorpaenidae) stranded on the Sicilian coast of the Strait of Messina (central Mediterranean Sea) are reported. Morphometric and meristic characters are given together with information on age data. The first underwater observations by remotely operated vehicle (ROV) in different Mediterranean areas contribute to improve current knowledge on distribution, behavior and habitat preference of this species. [ABSTRACT FROM AUTHOR]

This study focuses, for the first time, on the presence of plastic debris in the stomach contents of large pelagic fish ( Xiphias gladius , Thunnus thynnus and Thunnus alalunga ) caught in the Mediterranean Sea between 2012 and 2013. Results highlighted the ingestion of plastics in the 18.2% of samples. The plastics ingested were microplastics (<5 mm), mesoplastics (5–25 mm) and macroplastics (>25 mm). These preliminary results represent an important initial phase in exploring two main ecotoxicological aspects: (a) the assessment of the presence and impact of plastic debris on these large pelagic fish, and (b) the potential effects related to the transfer of contaminants on human health. [ABSTRACT FROM AUTHOR]

The feeding habits of the bathypelagic, non-migratory myctophidDiaphus metopoclampusfrom a shrimp fishing ground area in the Strait of Sicily were analyzed. Overall, 296 adult specimens ranging from 50.8 to 92.8 mm standard length (SL) (mean SL = 72.9 ± 8.0 mm) were collected during two trawl surveys (October 2010 and May 2011) and their stomach contents were examined in order to provide new information on prey composition and feeding strategy of this poorly known lanternfish. Differences in diet depending on fish size and time (daylight and night) were also checked by statistical analysis.D. metopoclampusis a zooplanktivorous predator, consuming mainly Crustacea, but also Mollusca, Polychaeta, Chaetognatha, Taliacea and Actinopterygii, and showing a moderate opportunistic feeding strategy.D. metopoclampuscan be ascribed to the group of acyclic predators, since it seems to be a non-migrator myctophid that feeds when prey are available. Statistical analysis indicated a size-related shift in the diet ofD. metopoclampusfrom smaller prey organisms (e.g, Metridinidae) to larger ones (e.g, Euphausiidae and Myctophidae). Some differences in prey composition between daylight and night were highlighted: euphausiids and myctophids were more abundant during night, Metridinidae during daylight. [ABSTRACT FROM AUTHOR]

This paper provides data on age, growth and diet of the bluemouth rockfish, Helicolenus dactylopterus dactylopterus (Scorpaenidae), central Mediterranean Sea. Estimated by otolith readings, ages ranged from 0 to 21 years. Parameters of the von Bertalanffy growth curves were Linf = 26.1 cm, k = 0.14 year−1, t 0 = −1.92 years and the growth performance index was Φ = 1.98. The weight-length relationship, W = 0.016 TL2.99; R2 = 0.99, described an isometric growth of the species. This species is a carnivore focusing mainly on the crustaceans Goneplax rhomboides and Lophogaster typicus. Statistical analysis showed an ontogenetic shift between small (4.0–6.3 cm) and larger fishes. Significant differences were also found in relation to the sampling depth. [ABSTRACT FROM AUTHOR]

Age and growth of pearly razorfish Xyrichtys novacula (Labridae) were determined in specimens caught on sandy bottoms by surrounding nets and lines (depth range 5–30 m) between January 2000 and December 2002. The study location was in the central Mediterranean Sea. For this purpose the annual growth increments (annuli) in sagittae were analysed. A total of 470 individuals of X. novacula, ranging from 49 to 200 mm TL (total length), were examined. Conversion between total length and standard length was calculated and represented by the relationship: . Edge analysis on otoliths was carried out to validate seasonality deposition. Seven age-classes were determined from 0+ to 6+. The linear relationship between maximum otolith length (OL) and TL was summarized in the equation . The estimated parameters of the von Bertalanffy growth function were L ∞ = 175.05 mm, k = 0.80 year−1, t0 =−0.73 years and the growth performance index calculated as ( Φ = 2.39). The length–weight relationship W = 0.0139 TL2.9326 described an isometric growth for the species in this area. [ABSTRACT FROM AUTHOR]

In order to assess feeding habits and trophic level of this species, we analysed the stomach content of 189 specimens collected monthly in the southern Tyrrhenian Sea (central Mediterranean Sea). The results showed that this species is a pelagic predator focusing on paralepidid fish and, more in detail, on two species such as Paralepis speciosa and Paralepis coregonoides. Among cephalopods, Heteroteuthis dispar was the most abundant prey item in the stomach contents, whereas among crustaceans the hyperidean amphipods Phrosina semilunata and Brachyscelus crusculum were the most representative species. In the present study, the diet of the albacore did not vary significantly throughout the period of study focusing, in the different season of catch, on the same prey species. Not even a size-related diet shift was observed, with a partitioning of the albacore sample into five size groups. Levin’s standardized index showed a low value indicating a limited trophic niche width, with a diet dominated by a limited number of taxa and then a specialist behaviour for this predator. The estimated TROPH value for T. alalunga resulted very similar to that calculated in Mediterranean Sea for other species as well as Xiphias gladius, Thunnus thynnus, Euthynnus alletteratus and Sarda sarda. Then, the albacore tuna can be considered as a top carnivore. [ABSTRACT FROM AUTHOR]

Illegal, unreported, and unregulated fishing for swordfish, mainly of immature specimens, are known to be the highest threats for this resource. This study provide a simple and useful tool for the maritime security service to recognize illegal landing sizes (<140 total length) of swordfish even when the specimens are deprived of their bills in order to elude the Italian law (Decreto del Presidente della Repubblica, /68). The purpose of the present paper is to reduce the illegal swordfish fishing in the Mediterranean Sea by supplying an alternative approach to calculate the total length, starting from the lower jaw fork length ( LJFL). In order to do this, the relationships of LJFL- FL and FL- TL of 214 specimens caught in the central Mediterranean Sea were examined by linear regression. Both linear models fit the data significantly at the P < 0.001 level. As the values of R2 were very close to 1, starting from the lower jaw-fork length of swordfish specimens and using these equations, it is possible to estimate the value of total length with a 99% approximation. [ABSTRACT FROM AUTHOR]

Marine litter pollution threatens marine ecosystems and biodiversity conservation, particularly on seafloors where all anthropogenic waste naturally sinks. In this study, we provide new information on the composition, density and origin of seafloor macrolitter as well as on plastic ingestion in deep-sea fish from bottom-trawling by-catch in the southern Tyrrhenian Sea. Plastic constituted the highest fraction of litter in terms of density (64 %) and weight (32 %) and was also retrieved in the gastrointestinal traits of Chlorophthalmus agassizi, Coelorhynchus coelorhynchus and Hoplosthethus mediterraneus. FT-IR spectroscopy analysis on the seafloor macrolitter and the ingested plastics revealed the presence of artificial polymers including PE, PET/polyester, PA widely used for food packaging, plastic bags and several common products, especially Single Use Plastic (SUP). These results underline how poor waste management schemes or their incorrect application strongly contribute to marine litter accumulation on seafloors and plastic ingestion in deep-sea fish., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Few studies focused on behaviour adaptations of organisms to marine litter (ML) pollution in Mediterranean Sea. This research, investigates on some behavior traits of Octopus vulgaris, focusing on the interaction with ML during the artisanal fishing activities by the bottom traps in a small coastal area of the southern Tyrrhenian Sea. For the first time, this pilot study uses an integrated approach based on the Fishermen Ecological Knowledge as well as the analysis of ML found in the traps. First assessment of plastic ingestion in this species are also reported. Plastic and metal were the predominant ML categories observed into the bottom traps. A total of 62 plastics, mainly small microplastics and fibres shaped, were ingested. The ML finding in the bottom traps suggests an interesting behavior of the common octopus regarding its interaction with ML, in fact, it seems to bring ML inside its dens, as a collector., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Plastic ingestion is one of the main impacts of marine litter on organisms. The occurrence of microplastics (MPs < 5 mm) in the stomachs of Mediterranean species was already reported in several studies. In this context, the present study aims to develop a new approach of digestion for the identification of MPs in the gastrointestinal tracts (GITs) of marine organisms. The new approach combines two digestion protocols, including potassium hydroxide (KOH) and nitric acid (HNO 3 ), to remove most organic and inorganic materials. This digestion allows recording small MPs that are difficult to find via routinely stomach content analysis and also to minimize the overestimation of the phenomenon trough the control of airborne contamination. The new approach was tested on a voracious pelagic opportunistic predator, the common dolphinfish, a fishery resource exploited in several Mediterranean areas. The results showed that a large amount of ingested meso- and microplastics, such as fragments or sheets, was recorded in GITs (F = 65.5 %). The FTIR analysis on litter samples allowed to identify polyethylene, polypropylene and polystyrene as dominant constituent polymers of microplastics. These results confirmed that our novel combined digestion protocol represents a reliable approach to detect MPs in opportunistic pelagic predators., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

The bluefin tuna, Thunnus thynnus, is a highly migratory and long-living fish at the top of the pelagic food web. As top predator, it plays a key role in the stability of marine food webs by exerting top-down control on its prey. The diet composition of bluefin tuna varies in relation to its growth, seasons and migratory patterns, making it difficult to evaluate spatial and temporal effects. This latter aspect is further complicated to be determined during the first months of life, when T. thynnus specimens have a rapid growth rate leading to changes in the trophic status. In this study, the potential collagen-related effects on δ 15 N and δ 13 C values were evaluated on the whole spine of adult tuna specimens collected in the central Mediterranean Sea. Obtained results showed non-significant differences between extracted and non-extracted collagen samples for δ 15 N in whole spine, allowing adopting the isotopic analysis both for annuli in the spine section of adults and for younger specimens, whose spine size does not permit the collagen extraction. Specifically, isotopic analysis of whole spine of the young of the year specimens, showed a rapid change in δ 15 N values with length, following an exponential model. For older specimens, δ 15 N values were higher and varied around a plateau, likely due to a higher specificity in the choice of prey and/or to change in the geographical location. Such variability was also mirrored in annuli of spines sections of adult tunas. As far as δ 13 C values are concerned, a strong collagen-related effect was evidenced, likely highlighting the influence of lipids. Consequently, δ 13 C analysis may be used only on adult specimens where collagen extraction is possible. This research also showed how isotopic analysis of both whole sample and sequence of annuli in the cross-section of dorsal spine might produce isotopic profiles useful to detect specific trophic dynamics along the bluefin tuna growth.

Mesopelagic organisms form huge biomass aggregations, supporting important pelagic trophic webs and several top predators. Although some studies on the occurrence, biology and ecology of these organisms are available, to date there are no investigations on their potential use for anticancer and antimicrobial biotechnological applications. The aim of this study was to screen extracts of seven mesopelagic species for possible anticancer (Lung cell line A549, skin cell line A2058, liver cell line HepG2, breast cell line MCF7 and pancreas cell line MiaPaca-2) and antibacterial (Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, the Gram-positive bacteria methicillin resistant/sensitive Staphylococcus aureus, and Mycobacterium tuberculosis) activities. Results showed that only two species were active, the lanternfish Myctophum punctatum and the Mediterranean krill Meganyctiphanes norvegica. In particular, M. punctatum showed strong activity against the A549 and MCF7 cells, while M. norvegica was more active against HepG2 cells. Regarding antibacterial assays, both species were active against methicillin resistant S. aureus. Fractionation and LC/MS dereplication of the fractions showed that the main compounds found in extracts of both species were EPA, DHA and ETA. For some of the detected compounds anticancer and/or antibacterial activity are already known, but this is the first time that such activities have been found for mesopelagic species.

Four specimens of Dosima fascicularis were collected from the Strait of Messina (Central Mediterranean Basin), representing the first record of the species from this locality, the second from Italian waters, and the most eastern indication of the species in the Mediterranean Sea.

The taxonomic, morphological and meristic features of a dark eel caught by commercial trawling off the Gulf of Patti, southern Tyrrhenian Sea, Mediterranean Sea, February 17, 2017, showed it to be a female Conger conger. Histological analysis of gonads demonstrated that the individual was in a post-spawning phase. X-ray radiographs showed widespread decalcification of skeleton and teeth loss, confirming a strong mobilisation of somatic energy reserves needed for reproductive development., (© 2019 The Fisheries Society of the British Isles.)

Citizen science programs carried out by volunteers are fundamental for the collection of scientific data on a wide spatial scale. From 2011 to 2018, 468 survey dives were conducted in 172 coastal locations of the Mediterranean Sea through Project AWARE's citizen science program, Dive Against Debris®. During the dives, information was collected on quantity, typology and distribution of seafloor litter in shallow coastal waters. Overall, the observed average density was 43.55 items/100 m 2 and plastic was largely the dominant material (55% of the total collected items) on basin scale. The most abundant seafloor litter items were plastic fragments with 9.46%, followed by beverage cans (7.45%). Single-use plastic items constituted 33% of the total marine litter. The results of the study can be used to inform policymakers of the European Community towards specific management action to contrast the marine litter in relation to the distribution of the recorded litter category., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

The distribution of floating litter in marine waters, influenced by currents and wind drag, often determines the dispersal of its encrusting fauna. In the present paper, we observed for the first time the colonization of rafting floats from abandoned, lost or derelict fishing gears (ALDFG) by the four protected deep-sea species: Errina aspera, Desmophyllum pertusum, Madrepora oculata Pachylasma giganteum. Overall, 41 floats, colonized by deep benthic species, were found stranded on the shore of the Sicilian coast of the Strait of Messina, between 2016 and 2019. Species composition, number and occurrence of colonizing organisms were analyzed. On the basis of the species composition (the association between E. aspera, P. giganteum and Megabalanus tulipiformis), the knowledge on their ecology, biogeography, path of local currents, it was possible to define that the area of origin of the most part of these fishing net floats was the Strait of Messina., (Copyright © 2019 Elsevier Ltd. All rights reserved.)