1. Presence of hepatitis E RNA in mussels used as bio-monitors of viral marine pollution.
- Author
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Donia D, Dell'Amico MC, Petrinca AR, Martinucci I, Mazzei M, Tolari F, and Divizia M
- Subjects
- Animals, Coliphages isolation & purification, Hepatitis A virus isolation & purification, Mediterranean Sea, Norovirus isolation & purification, Biosensing Techniques methods, Hepatitis E virus isolation & purification, Mytilus virology, RNA, Viral isolation & purification, Seawater virology, Water Pollution
- Abstract
Mussels (Mytilus galloprovincialis), collected from a harvesting area approved by European Community Regulation, were transplanted to four polluted sites located in the Northwestern Mediterranean area (Tuscany). They were used as bio-monitors to test the quality of the marine water pollution. At different times after the transplantation, mussels were withdrawn and tested for presence of phages and enteric viruses by molecular tests. 52.4% of the transplanted mussel samples were positive for at least one enteric virus. Hepatitis A virus (HAV) was identified in each site (17/37; 45.9%). Three samples were positive for hepatitis E virus (HEV) (8.1%) and two (5.4%) for norovirus (NoV) genogroup I. Coliphages and RYC 2056 phages were detected in all sites, while HSP 40 phages were detected in three sites. Results demonstrate the ability of transplanted mussels in accumulating and retaining different species of enteric microorganisms. Their utility as bio-monitor organisms enables testing for viral marine pollution., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2012
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