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Introduction: Osteoporosis is a multifactorial disease characterized by a low bone mineral density (BMD). Osteoporosis and the occurrence of fractures in postmenopausal women have been associated to the TaqI polymorphism in the vitamin D receptor (VDR) gene. Objective: To analyze the association of the different genotypes of TaqI polymorphism of the VDR gene with BMD in young Mexican women. Methods: Dual X-ray absorptiometry was carried out in 150 women aged 19 to 29 years in order to determine their total bone mineral density (tBMD) and dual BMD of the femur (dfBMD). DNA was extracted from peripheral blood to determine the genotype of the TaqI polymorphism in the VDR gene. The data obtained were analyzed by simple linear regression and ANOVA. Results: Mean tBMD was 1.096 ± 0.064 mean dfBMD was 0.960 ± 0.107 g/cm². The frequency of the TaqI polymorphisms was 57% (TT), 37% (Tt) and 6% (tt), the frequency of the alleles was 75% (T) and 25% (t). ThIs statistical analysis showed a lack of association between BMD and the genotypes of TaqI polymorphism in the VDR gene. Discussion and conclusions: These results suggest that may exist factors other than the TaqI polymorphism in the VDR gene contributing to BMD in young women from Northern Mexico. [ABSTRACT FROM AUTHOR]

Background: Osteoporosis is characterized by low bone mineral density (BMD), which is determined by an interaction of genetic, metabolic and environmental factors., Aim: To analyse the association between two polymorphisms of VDR as well as their haplotypes with BMD in post-menopausal Maya-Mestizo women., Subjects and Methods: This study comprised 600 post-menopausal Maya-Mestizo women. A structured questionnaire for risk factors was applied and BMD was assessed at the lumbar spine (LS) and total hip (TH) by dual-energy X-ray absorptiometry. DNA was extracted from blood leukocytes. Two single-nucleotide polymorphisms of VDR (rs731236 and rs2228570) were studied using real-time PCR allelic discrimination for genotyping. Differences between the means of the BMDs according to the genotype were analysed with covariance. Haplotype analysis was conducted., Results: TT genotype of rs731236 of VDR had higher BMD at total hip and femoral neck (FN), and one haplotype formed by the two polymorphisms was associated with only TH-BMD variations. This difference was statistically significant after adjustment for confounders. The genotype of rs2228570 of VDR analysis showed no significant differences with BMD variations., Conclusion: The results showed that the TT genotype of rs731236 of VDR and one haplotype formed by rs731236 and rs2228570 polymorphisms were associated with higher BMD at TH and FN.

Background: Since osteoporosis is a complex disease characterized by low bone mineral density (BMD), which is determined by an interaction of genetics with metabolic and environmental factors, the aim of this study was to analyze the possible association among one polymorphism of VDR and two polymorphisms of ESR1; as well as their haplotypes with BMD in postmenopausal Mexican-mestizo women., Methods: We studied 742 postmenopausal Mexican-mestizo women. A structured questionnaire for risk factors was applied and BMD was measured in the lumbar spine and total hip by dual-energy X-ray absorptiometry. DNA was obtained from blood leukocytes. One polymorphism of VDR (rs11568820) and two of ESR1 (rs2234693 and rs9340799) were studied. Real-time PCR allelic discrimination was used for genotyping. The differences between the means of the BMDs according to genotype were analyzed with covariance. Hardy-Weinberg equilibrium was tested. Pairwise linkage disequilibrium between single nucleotide polymorphisms was calculated by direct correlation r(2); haplotype analysis was conducted., Results: Rs9340799 of ESR1 and one haplotype formed by the two polymorphisms of the ESR1 were significantly associated with FN-BMD variations. Moreover, analysis of the genotype of rs11568820 of VDR and the rs2234693 of ESR1 showed no significant differences with BMD variations., Conclusions: Our results showed that rs9340799 and one haplotype of ESR1 were significantly associated with BMD only at the femoral neck and this association remained after adjusting for covariates., (Copyright © 2013 Elsevier B.V. All rights reserved.)