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Current social conditions require more accurate methods to determine the age of people, either living or deceased. The aim of this study was to determine chronological age by measuring the mineralization of third molars. Volunteers (n = 137), aged between 14 and 22 years and of either gender, were recruited from north-eastern Mexico. Sampling was probabilistic and at random. The mineralization stage of third molars was evaluated with the use of multi-slice helical computed tomography and the Mincer-modified Demirjian amended scale. The correlation coefficient, mean difference and linear estimation were determined. There was a linear correlation between age and the mineralization of third molars. Third molars of adults had a degree of mineralization above Demirjian stage F, whereas the third molars of minors had stages between A and F. Hence, a method was obtained to determine the chronological age from the evaluation of dental mineralization of third molars by multi-slice helical tomography. These results are useful as a diagnostic support in forensic dentistry. [ABSTRACT FROM AUTHOR]