1. SHV-type Extended-spectrum ß-lactamase (ESBL) are encoded in related plasmids from enterobacteria clinical isolates from Mexico.
- Author
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Garza-Ramos, Ulises, Martínez-Romero, Esperanza, and Silva-Sánchez, Jesús
- Subjects
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BETA lactamases , *ENTEROBACTERIACEAE , *PLASMIDS , *CEPHALOSPORINS - Abstract
Objective: In this work we report the molecular characterization of β-lactam antibiotics resistance conferred by genes contained in plasmids from enterobacteria producing extended-spectrum β-lactamases (ESBL). Material and Methods: Fourteen enterobacterial clinical isolates selected from a group of strains obtained from seven different hospitals in Mexico during 1990-1992 and 1996-1998 were analyzed at the Bacterial Resistance Laboratory (National Institute Public Health, Cuernavaca). Molecular characterization included PFGE, IEF of β-lactamases, bacterial conjugation, PCR amplification and DNA sequencing, plasmid extraction and restriction. Results: Isolates were genetically unrelated. ESBL identified were SHV-2 (5/14) and SHV-5 (9/14) type. Cephalosporin-resistance was transferable in 9 of 14 (64%) clinical isolates with only one conjugative plasmid, DNA finger printing showed a similar band pattern in plasmids. Conclusions: The dissemination of cephalosporin resistance was due to related plasmids carrying the ESBL genes. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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