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The objective was to evaluate the antioxidant and biological potential of eight freeze-dried berry varieties of southern Jalisco using in silico and in vitro approaches. Fourteen tentative phenolic compounds were identified in berries by ESI-QToF, including anthocyanins, phenolic acids, flavanols and flavonols. In silico assays of phytochemicals in the berry inhibiting enzymes related to obesity and diabetes showed predicted binding energy interactions (ranging from −5.4 to −9.3 kcal/mol). Among the cultivars, antioxidant potential for DPPH IC50 ranged from 1.27 to 3.40 mg/mL, ABTS IC50 from 2.26 to 7.32 mg/mL and nitric oxide (NO) inhibition IC50 from 4.26 to 11.07 mg/mL. The potential to inhibit α-amylase IC50 ranged from 4.02 to 7.66 mg/mL, α-glucosidase IC50 from 0.27 to 4.09 mg/mL, lipase IC50 from 1.30 to 4.82 mg/mL and DPP-IV IC50 from 1.36 to 3.31 mg/mL. Blackberry cultivars from the southern Jalisco region showed outstanding biological potential compared to other evaluated berries and could be used in the formulation of functional foods in the prevention of noncommunicable diseases. [ABSTRACT FROM AUTHOR]

The Mayan cichlid (Cichlasoma urophthalmus), is a freshwater fish of the Cichlidae family, native to the south east of Mexico, it has potential for extensive cultivation due to its excellent meat quality and local demand. However, the nutrigenomic aspects and molecular expression of genes involved in its digestion that could contribute to its growth during the larval stage are unknown. Some enzymes such as Pepsin (PEP), pancreatic such as trypsin (TRY), Chymotrypsin [CHY] and intestinal enzymes such as Leucine aminopeptidase [ANPEP], function during larval ontogenesis and activity patterns of the key factors involved in the digestive system. For this study, 30–50 larvae were collected, which were processed with mRNA extraction techniques and cDNA synthesis by RT‐PCR using qPCR oligos designed from Oreochromis niloticus, measuring molecular expression during the initial ontogeny of C. urophthalmus. The results obtained show the molecular expression of these enzymes occurs through epigenetic factors such as changes from endogenous to exogenous feeding, presenting the activation of genes for the formation of organs of the digestive tract that allow the secretion of specific enzymes for processing and digestion of food, that can activate these genes to increase the digestion efficiency and the optimal use of nutrients. [ABSTRACT FROM AUTHOR]