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Objective: To investigate the effect of Gubi Tongxiao granules on vascular formation and cell apoptosis in the process of glucocorticoid-induced femoral head necrosis. Methods: Thirty experimental New Zealand rabbits were randomly divided into groups. Except the control group, the animal model of steroid-induced femoral head necrosis was established by lipopolysaccharide combined with glucocorticoids. After successful modeling, the experimental group was given Gubi Tongxiao granules intragastric treatment, the model and control group were given the same amount of normal saline intragastric treatment, once a day, for 8 weeks, the experimental animals were sacrificed, the bilateral femoral head of each group was taken out for hematoxylin-eosin staining, and protein CD34,CYR61 and VEGF were immunohistochemical staining localization. TUNEL staining was used to observe the apoptosis of femoral head cells. The expression levels of apoptosis proteins Bax and Bcl-2 were detected by WB. The expression of VEGF eNOs Bax and Bcl-2 genes was analyzed by RT-qPCR.Results: Gubi Tongxiao granules reduced the number of hollow bone lacunae and apoptotic positive cells(P<0.01), and up-regulated the expressions of CD34, CYR61 and VEGF in femoral head tissue. Compared with control group, the expression of pro-apoptotic protein Bax increased and the expression of anti-apoptotic protein Bcl-2, VEGF and eNOs mRAN decreased significantly in the model group (P<0.01), the opposite results were obtained in the experimental group after Gubi Tongxiao granule intervention (P<0.05). Conclusion: Gubi Tongxiao granules can improve the expression of angiogenic genes and regulate apoptosisrelated proteins in the bone tissue of rabbit model with steroid-induced necrosis of femoral head, promote angiogenic differentiation and inhibit apoptosis, and thus achieve the effect of treating steroid-induced necrosis of femoral head. [ABSTRACT FROM AUTHOR]