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The aim of this study was to investigate the effect of taxifolin on spermatological parameters of rabbit semen during liquid storage (0, 6, 12, and 24th h). In this study, eight adult New Zealand male rabbits were used as semen donors. Semen was taken with the aid of artificial vagina three times a week. Ejaculates collected from bucks were evaluated and pooled at 37oC. Semen samples were divided into four groups then were diluted with Tris (K-Tris), Tris + 10 µl taxifolin (10 TT), Tris + 100 µl taxifolin (100 TT) and Tris + 500 µl taxifolin (500 TT). The diluted semen samples were liquid stored at 4°C up to 24th h. After the storage period, some spermatological parameters (the percentage of motility, acrosome - total spermatozoa abnormality and HOST) were analyzed. Experimental studies were replicated 10 times. As a result of the study, the percentage of abnormal spermatozoa increased and plasma membrane integrity and motility rate decreased at the end of 24th h in all experimental groups. When compared to the control group data, 100 µl taxifolin addition gave the highest motility rate at 6th (82.50%), 12th (80%) and 24th (77.50%) hour (P <0.001). At the 24th hour of liquid storage, 100 µl taxifolin addition was found to protect statistically significantly the integrity of acrosomal structure and plasma membrane compared to other groups. In addition, it was determined that the percentage of acrosome (7%) and total spermatozoa abnormality (8.50%) was low in the 100 TT group when compared to other groups (P <0.001). In conclusion, it was found that addition of 100 µl taxifolin plays effective protective role on short-term storage of rabbit semen. [ABSTRACT FROM AUTHOR]