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Camel abscess is one of the important diseases that can be caused by pyogenic bacteria. Abscess were mainly reported on the dromedary camel of the Nile Delta of Egypt, but rarely reported on Bactrian camel. The purpose of this study is to identify the pathogens isolated from the samples of camel abscess. The scanning electron microscope was applied for pathogenic morphological study. The 16S rRNA genes amplification and sequencing, and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) method were employed to identify the bacteria. In addition, drug sensitivity tests were performed for the potential prevention and control of the disease. The results showed that two bacterial strains of Staphylococcus were isolated. Amplification of 16S rRNA and construction of phylogenetic tree demonstrated that they were Staphylococcus aureus (S. aureus) and Staphylococcus sciuri (S. sciuri). The results of MALDI-TOF MS confirmed that the causative bacteria causing camel abscess were S. aureus and S. sciuri. Drug sensitivity tests showed that S. aureus was resistant to Amoxicillin, Penicillin G, Erythromycin, Linezolid, Clindamycin, and Chloramphenicol, and was moderately resistant to Rifampicin, Doxycycline, and Tetracycline, but was sensitive to seven other antibiotics. S. sciuri was resistant to Cefotaxime, but sensitive to fifteen other antibiotics. This study is the first report on S. sciuri infected camels and provides a scientific basis for selecting antibiotics that are effective for treating camel abscess. [ABSTRACT FROM AUTHOR]