Search

Pineapple weed (Matricaria discoidea DC.) is a widespread plant in Europe and North America. In ethnomedicine, it is well-known for its anti-inflammatory and spasmolytic activities. The aim of this research was to develop novel methods of M. discoidea processing to obtain essential oil and dry extracts and to investigate their phytochemical compositions. Moreover, the molecular docking of the main substances and the in vivo studies on their soporific and analgesic activities were conducted. The essential oil and two dry extracts from M. discoidea were prepared. A total of 16 phenolic compounds (seven flavonoids, seven hydroxycinnamic acids, and two phenolic acids) in the dry extracts were identified by means of UPLC-MS/MS. In the essential oil, nine main terpenoids were identified by gas chromatography (GC). It was shown that phenolic extraction from the herb was successful when using 70% ethanol in a triple extraction method and at a ratio of 1:14–1:16. The in vivo studies with rodents demonstrated the analgesic activity of the M. discoidea extracts and improvements in the sleep of animals. The dry extracts of M. discoidea did not show any toxicity. The molecular docking analysis showed a high probability of COX-1,2 inhibition and NMDA receptor antagonism by the extracts. [ABSTRACT FROM AUTHOR]

The ecological drivers of herbaceous layer composition and diversity in deciduous forests of eastern North America are imperfectly understood. We analyzed the herbaceous layer, across the growing season, in a central Appalachian old-growth forest to examine dynamics, diversity, and relationships to resource gradients. We found clear variation in herb species composition over the growing season. We identified intermingled resource gradients, including soil nutrients, light availability, and topography, that were related to herbaceous composition. We found that herb layer diversity was different among previously identified tree communities, but was not variable over the growing season. We identified a unimodal relationship between diversity and productivity in the herb flora that held throughout the growing season despite changing composition and levels of productivity. Diversity and distributions in the herbaceous community of our study site are linked to a complex of resource gradients. [ABSTRACT FROM AUTHOR]

Reintroduction of periodic dormant-season fire and overstory thinning are currently being employed for forest ecosystem management in deciduous forests of eastern North America. These manipulations usually alter the flux of light and the availability of soil nutrients to the perennial herbaceous plants that dominate the understory. We utilized Bayesian statistical methods to examine the effects of prescribed burning (B) and the combination of burning and overstory thinning (T+B) on the morphology, seed production, and early establishment of Desmodium nudiflorum (L.) DC. (Fabaceae) in mixed-oak (Quercus spp.) forests in southern Ohio. During the fourth growing season after the first fire, plants from thinned and burned (T+B) plots were 62% larger than plants from control plots (C). Both burning alone (B) and T+B treatments decreased specific leaf area (SLA). T+B also resulted in significantly decreased root mass ratio (RMR), and increased leaf mass ratio (LMR), and specific root length (SRL). During the first growing season after a second fire, both B and T+B resulted in significantly increased plant biomass, LMR, individual seed mass, and total seed production, as well as decreased SLA and plant height; in contrast, neither B nor T+B had significant impacts on leaf area ratio or seedling establishment. Prescribed fire, especially when combined with thinning, can result in increases in total biomass, seed size, and seed production, and hence enhance the fitness of this perennial herb in these mixed-oak forests. [ABSTRACT FROM AUTHOR]