1. HPLC–MS analyses and bioactivities of novel chemicals in Devil’s club (Oplopanax horridus (Sm.) Miq.)
- Author
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You, Qi, Chen, Feng, Ni, Hui, Wang, Xi, Jiang, Yueming, and McCoy, Joe-Ann H.
- Subjects
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CHRONIC disease treatment , *HIGH performance liquid chromatography , *TRADITIONAL medicine , *LIQUID-liquid extraction , *PLANT roots , *PLANT extracts - Abstract
Abstract: Devil’s club (Oplopanax horridus (Sm.) Miq.) has traditionally been used as a folk medicine by native North Americans for the treatment of various chronic diseases. A methanolic extract and its sub-fractions by liquid–liquid re-extraction, i.e., chloroform (CHCl3), ethyl acetate (EtOAc), n-butanol (BuOH), and water fractions, were analysed by their anti-cancer activity with in vitro cell proliferative bioassays, and their antioxidant capacities in terms of the determination of total phenolic content (TPC), ORAC value, and DPPH free radical scavenging activity. The whole extract of the dried root contained high TPC and possessed strong ORAC and DPPH radical scavenging activities. In addition, the extract exhibited a strong anti-proliferative ability against HT-29 cancer cells, e.g., with an inhibitive rate of 56.5% with a 0.2-mg/mL extract. Further investigation by HPLC–UV–MS identified significant bioactive phenolic compounds in the chloroform fraction, including gallic acid, caffeic acid, 4-O-feruloylquinic acid, 5-O-feruloylquinic acid, ferulic acid, methyl feruloylquinate, methyl ferulate, and quercetin. These results suggested that the associated bioactivity of the plant might result from the contribution of phenolic compounds. [Copyright &y& Elsevier]
- Published
- 2012
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