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Soil organic carbon (SOC) plays a critical role in major ecosystem processes, agriculture, and climate mitigation. Accurate SOC predictions are challenging due to natural variation, as well as variation in data sources, sampling design, and modeling approaches. The goal of this study was to (i) understand SOC stock distribution due to land use (restored prairie grass—PG; lawn grass—LG; and forest—F), and local topography, and (ii) assess the scalability of SOC stock predictions from the study site in North Carolina (Lat: 36°7′ N; Longitude: 80°16′ W) to the geographic extension of the Fairview soil series based on the US Soil Survey Geographic (gSSURGO) database. Overall, LG had the highest SOC stock (82 Mg ha−1) followed by PG (79 Mg ha−1) and forest (73.1 Mg ha−1). SOC stock decreased with the depth for LG and PG, which had about 60% concentrated on the surface horizon (0–23 cm), while forest had only 40%. The differences between measured SOC stocks and those estimated by gSSURGO and modeled based on land use for the Fairview series extent were comparable. However, subtracting maps of the uncertainty predictions based on the 90% confidence interval (CI) derived from the measured values and estimated gSSURGO upper and lower values (an estimated CI) resulted in a range from −17 to 41 Mg ha−1 which, when valued monetarily, varied from USD 33 million to USD 824 million for the Fairview soil series extent. In addition, the spatial differences found by subtracting the gSSURGO estimations from measured uncertainties aligned with the county administrative boundaries. The distribution of SOC stock was found to be related to land use, topography, and soil depth, while accuracy predictions were also influenced by data source. [ABSTRACT FROM AUTHOR]