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The Mazama gouazoubira population density was estimated in the Mburucuyá National Park (Corrientes, Argentina). The assessment methods used where: strip transects sampling. Samplings were performed from June 2008 to February 2011, distributed at summer and winter in daytime hours (0600 - 1000 h) and night (1900 - 2200 h) in three habitats: mesophilous forests, “pajonales" (tall grasslands) and yatay palm groves. A number of 186 records were obtained after 344.76 km traveled. The calculated population size was 430 individuals (CI = 324-548), based on available habitat area in the park (62.3 km2), and an estimated average density of 6.9 ± 0.9 brocket deer/km2. Density varied significantly in each area: 11.2 ± 1.4 ind/km2 in mesophilous forest, 3.5 ± 1.1 ind/km2 in pajonales, and 5.5 ± 1.5 ind/km2 in yatay palm groves. Results showed Mazama gouazoubira´s higher affinity for mesophilous forests. Population density estimates of this species will allow monitoring and future studies in the northeastern region of Argentina. [ABSTRACT FROM AUTHOR]