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A significant proportion of academic spin-offs (ASOs) are founded by entrepreneurial teams (ETs). Yet little is known about how these ETs are formed or the role of technology transfer offices (TTOs) in this formation process. This article examines whether and how TTOs affect the formation of academic spin-off entrepreneurial teams (ASO-ETs). To this end, we study in detail the formation of seven ETs behind life-science ASOs developed in one region in Norway. Our findings show that ASO-ETs followed different paths of formation, partly mirroring the organization of the TTOs. We further identify four different roles played by TTOs, two direct and two indirect, that shape the formation of these ETs. Based on organization imprinting theory, we contribute to the team entrepreneurship literature by developing a new framework showing how TTOs imprint the formation of ETs in ASO settings. [ABSTRACT FROM AUTHOR]