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The roles mobile animals and abiotic processes play as vectors for resource transfers between ecosystems ("subsidies") are well studied, but the idea that resources from animals with limited mobility may be transported across boundaries through intermediate taxa remains unexplored. Aquatic plants ("macrophytes") are globally distributed and may mediate transfers of aquatic‐derived nutrients from aggregations of aquatic animals to terrestrial ecosystems when consumed by terrestrial herbivores. We used mesocosms (94 × 44 cm) to test whether aquatic animal‐generated biogeochemical hotspots increase growth and nutrient content in macrophytes using the macrophyte Justicia americana and freshwater mussels. Justicia americana biomass production increased and belowground biomass allocation changed with increasing mussel density. At high mussel density, water‐column phosphorus increased and carbon:phosphorus ratios in J. americana tissues decreased. We deployed motion‐sensing cameras to explore herbivory on J. americana growing along the margins of the Kiamichi River, Oklahoma, and documented feeding by large mammals (Odocoileus virginianus, Sus scrofa, and Bos taurus). Thus, biogeochemical hotspots generated by aquatic animal aggregations can promote macrophyte production that subsequently is transferred to terrestrial animals. More broadly, this suggests that reductions in aquatic animal biomass may have bottom‐up impacts that indirectly affect terrestrial ecosystems via plant–animal interactions bridging ecosystem boundaries. [ABSTRACT FROM AUTHOR]

Biomass is often used to scale the contributions of individuals and their functional traits to a community or ecosystem. However, accurate biomass measurements can require destructive sampling, which is detrimental to long-lived organisms such as unionid mussels. We amassed a database of 6684 measurements of length and soft tissue dry mass (STDM) or shell dry mass (SHDM) from 43 species of unionid mussels to reduce the need for destructive sampling. We used these data to produce regression equations that relate maximum shell length to mass (either STDM or SHDM) at 3 taxonomic levels: family, phylogenetic tribe, and species. For 2 widely-distributed unionid species, Amblema plicata and Elliptio complanata , we present length–STDM regression equations from 6 waterbodies and basins. We also used bootstrapping resampling at the family level to develop a universal regression equation for unionid mussels. We compared models within all 3 taxonomic levels to determine if multiplicative (log–log transformation) or additive (non-linear parameter estimation) error structures provided better fits for biomass prediction. Models with multiplicative errors best fit length–STDM data at the family level (STDM = 6.63 × 10−6 × L max2.89 × 1.13; r 2 = 0.94), for 83% of tribes (n = 5, average r 2 = 0.95 ± 0.03), and for 90% of species (n = 33, average r 2 = 0.93 ± 0.07). For length–SHDM, models with multiplicative errors best fit the family level (SHDM = 2.98 × 10−4 × L max2.98 × 1.32; r 2 = 0.86), all tribes (n = 5, average r 2 = 0.88 ± 0.13), and all species (n = 27, average r 2 = 0.94 ± 0.09). Models with multiplicative errors also provided the best fit (r 2 > 0.76) for our 2 wide-ranging species, A. plicata and E. complanata. Finally, we present a case study based on data collected from 19 river sites in Alabama and Oklahoma, USA, to determine the performance of our power relationships (bootstrap resampling versus length–STDM regressions). In both river systems, tribe- and species-specific equations improved the prediction of unionid STDM over the family-level regression by 2–20%. Finer taxonomic resolution equations produce more accurate mass predictions, but where accurate taxonomic identifications are lacking, our family-level regression for STDM will produce acceptable estimates, which are key parameters when estimating mussel contributions to ecosystem services. Our study provides a toolkit that will allow scientists and managers to non-destructively quantify biomass (with uncertainty) of freshwater unionid mussels for secondary production, ecosystem function, and services estimates. [ABSTRACT FROM AUTHOR]