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Abstract: Background: Increased awareness of organ donation/transplantation has been found to have a positive influence on organ donation rates. One Life … Many Gifts is a curriculum resource in Ontario, Canada, to educate and raise awareness for senior secondary school students about the importance of organ and tissue donation and transplantation. Teachers administered pre- and post- attitudinal surveys to senior secondary school students to evaluate changes in their attitudes toward organ and tissue donation and transplantation. In this study, the effect of the One Life … Many Gifts educational intervention on changing the attitudes and awareness of organ donation and transplantation of senior secondary students was assessed. Methods: Mann–Whitney U tests were used to compare the pre- and post-surveys as a whole and then again for the data from individual schools. Kruskal–Wallis H-tests were used to compare data between schools. Results: A total of 1832 pre- and 1440 post-educational surveys returned to Trillium Gift of Life Network were assessed in the 2008–2009 academic year. Overall, comparison of all pre- and post-educational program survey data showed significant improvement in the students'' attitudes toward organs and tissue donation and transplantation (P = .00625). Conclusion: The One Life … Many Gifts program made an impact on changing the attitudes toward and awareness of organ donation among high school students in Ontario. Evaluation of its impact on donor registration and organ donation among the students and their parents is complex and remains to be seen. [Copyright &y& Elsevier]