1. Investigation of α‐Synuclein and Amyloid‐β(42)‐E22Δ Oligomers Using SiN Nanopore Functionalized with L‐Dopa.
- Author
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Abrao‐Nemeir, Imad, Bentin, Jeremy, Meyer, Nathan, Janot, Jean‐Marc, Torrent, Joan, Picaud, Fabien, and Balme, Sebastien
- Subjects
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ALPHA-synuclein , *SILICON nitride , *DIELECTRIC breakdown , *TECHNOLOGICAL innovations , *SINGLE molecules , *OLIGOMERS - Abstract
Solid‐state nanopores are an emerging technology used as a high‐throughput, label‐free analytical method for the characterization of protein aggregation in an aqueous solution. In this work, we used Levodopamine to coat a silicon nitride nanopore surface that was fabricated through a dielectric breakdown in order to reduce the unspecific adsorption. The coating of inner nanopore wall by investigation of the translocation of heparin. The functionalized nanopore was used to investigate the aggregation of amyloid‐β and α‐synuclein, two biomarkers of degenerative diseases. In the first application, we demonstrate that the α‐synuclein WT is more prone to form dimers than the variant A53T. In the second one, we show for the Aβ(42)‐E22Δ (Osaka mutant) that the addition of Aβ(42)‐WT monomers increases the polymorphism of oligomers, while the incubation with Aβ(42)‐WT fibrils generates larger aggregates. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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