1. 6,6'-Bieckol inhibits adipocyte differentiation through downregulation of adipogenesis and lipogenesis in 3T3-L1 cells.
- Author
-
Kwon TH, Wu YX, Kim JS, Woo JH, Park KT, Kwon OJ, Seo HJ, Kim T, and Park NH
- Subjects
- 3T3-L1 Cells, Animals, Anti-Obesity Agents adverse effects, Anti-Obesity Agents chemistry, Anti-Obesity Agents isolation & purification, Benzofurans adverse effects, Benzofurans chemistry, Benzofurans isolation & purification, Benzofurans pharmacology, Carbon-Carbon Ligases antagonists & inhibitors, Carbon-Carbon Ligases genetics, Carbon-Carbon Ligases metabolism, Cell Survival drug effects, Dioxins adverse effects, Dioxins chemistry, Dioxins isolation & purification, Fatty Acid Synthase, Type I antagonists & inhibitors, Fatty Acid Synthase, Type I genetics, Fatty Acid Synthase, Type I metabolism, Mice, Molecular Structure, PPAR gamma antagonists & inhibitors, PPAR gamma genetics, PPAR gamma metabolism, Pacific Ocean, Phaeophyceae chemistry, Republic of Korea, Seaweed chemistry, Stereoisomerism, Adipogenesis drug effects, Anti-Obesity Agents pharmacology, Dioxins pharmacology, Down-Regulation drug effects, Lipid Metabolism drug effects
- Abstract
Background: Brown algae have been used for their nutritional value as well as a source of bioactive compounds with antioxidant, anti-inflammatory, antimicrobial and anti-obesity effects. Obesity is an important condition implicated in various diseases, including diabetes, hypertension, dyslipidemia and coronary heart disease. However, anti-obesity effects of Eisenia bicyclis remain unknown., Results: We investigated the anti-obesity effects of 6,6'-bieckol, 6,8'-bieckol, 8,8'-bieckol, dieckol and phlorofucofuroeckol A isolated from E. bicyclis. Anti-obesity activity was evaluated by examining the inhibition of differentiation of 3T3-L1 adipocytes and the expression of peroxisome proliferator-activated receptor γ (PPARγ), CCATT/enhancer-binding protein α (C/EBPα) and sterol regulatory element binding protein-1c (SREBP-1c) at the mRNA and protein level. Differentiated 3T3-L1 cells were treated with the purified phlorotannins at concentrations of 10, 25 and 50 µg mL(-1) for 8 days. The results indicated that the purified phlorotannins suppressed the differentiation of 3T3-L1 adipocytes in a dose-dependent manner, without toxic effects. Among the five compounds, 6,6'-bieckol markedly decreased lipid accumulation and expression levels of PPARγ, C/EBPα, SREBP-1c (mRNA and protein), and fatty acid synthase and acyl-coA carboxylase (mRNA)., Conclusion: These findings suggest that E. bicyclis suppressed differentiation of 3T3-L1 adipocyte through downregulation of adipogenesis and lipogenesis., (© 2014 Society of Chemical Industry.)
- Published
- 2015
- Full Text
- View/download PDF