1. Sol-gel immobilisation of methyl parathion degrading bacteria isolated from agricultural areas of Pakistan.
- Author
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Idrees, Muhammad, Akber Shah Eqani, Syed Ali Musstjab, and Bokhari, Habib
- Subjects
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SOL-gel processes , *IMMOBILIZATION stress , *METHYL parathion , *BIOREMEDIATION , *AGRICULTURE , *SOIL microbiology , *SOIL degradation - Abstract
The following areas are discussed in this paper: immobilisation of bacterial consortium in sol-gel; methyl parathion degradation and bioremediation applications; evaluation of indigenous bacterial isolates of contaminated soils. Bacterial strains were isolated from agricultural areas of Pakistan which were contaminated with methyl parathion. A bacterial consortium of seven (out of 64) Enterobacteriaceae isolates including Citrobacter, Enterobacter and Proteus vulgaris capable of degrading methyl parathion (enzyme activity ranging 410-675 mU mL-1 for individual isolates and 982 mU/mL for consortium) was selected and subsequently immobilised in tetraethyl orthosilicate (TEOS) and sodium-silicate-based sol-gel matrices. Cell viability of suspended and immobilised bacterial consortium was monitored using a minimal salt medium supplemented with methyl parathion. The results indicate that sol-gel immobilisation can be helpful to increase the shelf life of methyl parathion degrading bacterial strains along with preservation of biological activity for bioremediation applications in field. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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