1. POTENTIAL DISSEMINATION OF ANTIMICROBIAL RESISTANCE FROM SMALL SCALE POULTRY SLAUGHTERHOUSES IN PAKISTAN.
- Author
-
WASEEM, H., ALI, J., JAMAL, A., and ALI, M. I.
- Subjects
COMPARATIVE genomics ,MOBILE genetic elements ,CHICKENS ,SLAUGHTERING ,BROILER chickens ,POULTRY ,GUT microbiome - Abstract
The importance of environmental reservoirs as a source from which antimicrobial resistance emerges and subsequently transferred is already established. To evaluate the role of small scale poultry slaughterhouses in resistance dissemination in Pakistan, a comparative genomic analysis of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) in the gut microbiome of broiler and household chickens (Gallus gallus domesticus) was performed. An array of 52 qPCR primers targeting the 16S rRNA gene, ARGs and MGEs was used in the experiment to analyse the abundance of selected genes in collected samples. A total of 22 ARGs and 7 MGEs were detected in all samples. The detection frequency of specific gene classes and diversity of genes was found to be higher in broiler as compared to household chicken. Sul1 was the most abundant ARG with the highest percent relative abundance (2.4%). Total percent relative abundance of ARGs in broiler chicken was found to be significantly (p < 0.05) greater than household chicken fecal samples. A significant linear correlation (R² = 0.89) was found between relative abundances of int1 and total ARGs. The clustering and correlation of selective ARGs with MGEs has implied that small scale poultry slaughterhouses can be a potential source for the dissemination of ARGs to other non-resistant environmental and/or clinical bacteria. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF