1. First Description of bla NDM-7 Carried on an IncX4 Plasmid in Escherichia coli ST679 Isolated in Spain.
- Author
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Espinal P, Miró E, Segura C, Gómez L, Plasencia V, Coll P, and Navarro F
- Subjects
- Aminoglycosides pharmacology, Anti-Bacterial Agents pharmacology, Ciprofloxacin pharmacology, Drug Resistance, Multiple, Bacterial, Escherichia coli drug effects, Escherichia coli enzymology, Escherichia coli isolation & purification, Escherichia coli Infections drug therapy, Escherichia coli Infections microbiology, Escherichia coli Proteins metabolism, Gene Expression, Humans, Male, Microbial Sensitivity Tests, Middle Aged, Multilocus Sequence Typing, Pakistan, Plasmids chemistry, Spain, Travel, beta-Lactamases metabolism, beta-Lactams pharmacology, Escherichia coli genetics, Escherichia coli Proteins genetics, Genes, Bacterial, Plasmids metabolism, beta-Lactamases genetics
- Abstract
This study describes the molecular characterization of an NDM-7 carbapenemase-producing Escherichia coli strain Ec188, recovered from a rectal swab of a male patient who had travelled to Pakistan before his hospitalization at the Hospital del Mar in Barcelona, Spain. The Ec188 isolate, assigned to a new multilocus sequence type ST679, was resistant to all beta-lactams, aminoglycosides (gentamicin, tobramycin, and with reduced susceptibility to amikacin), and ciprofloxacin. The bla
NDM-7 gene was located on a 50 kb IncX4 plasmid (pEc188-NDM7), both in the original and transconjugant strains. In addition, blaCTX-M-15 was located on a 150 kb IncFIA plasmid and blaCMY-2 on a 95 kb undetermined plasmid type, only in the wild-type strain. The immediate genetic surroundings of blaNDM-7 included the bleo, trpf, and dsbC genes, and it was flanked by the insertion sequences IS26 and ISAba125, which appeared interrupted by IS5. The res and parA genes were found in the same orientation downstream of the IS26 element. To our knowledge, this is the first report of an NDM-7-carbapenemase carried on an IncX4 plasmid, as well as the first E. coli strain belonging to ST679 harboring an NDM β-lactamase, possibly associated with previous travel to Pakistan. In addition, this study highlights the dissemination of NDM variants accompanied by IncX-type plasmids.- Published
- 2018
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