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Two different weight groups of farmed Labeo rohita were collected from fish hatchery, Satiana Road Faisalabad, Pakistan. Pesticide residues were determined by high-performance liquid chromatography (HPLC). HPLC analysis showed that seven pesticide residues (α-endosulfan, DDE, parathion methyl, isoproturon, atrazine, carbofuran, and deltamethrin) were detected in L. rohita in weight group W 1.Six pesticide residues (α-endosulfan, parathion methyl, isoproturon, atrazine, carbaryl, and carbofuran) were found in L. rohita weight group W 2. The overall comparison between two weight groups of L. rohita showed that maximal concentration of pesticide residues were observed in underweight group W 2 compared to W 1 weight group. [ABSTRACT FROM AUTHOR]

Maternal oxidative stress during pregnancy may impair fetal growth and help in the development of diseases in adulthood. The aim of current study was to assess total oxidation status (TOS), related parameters and their relationship to DNA damage (%) and homocysteine level in normal pregnant women in low-income participants. In a cross-sectional study healthy women were grouped as normal, while age matched nulliparous and singleton pregnancies were included for first, second and third trimester groups. TOS (P<0.01), melanodialdehyde (MDA) (P<0.001), aspartate aminotransferase (AST) (P<0.01), triiodothyronine (T3) (P<0.01), thyroxine (T4) (P<0.01), and homocysteine (P<0.001), in pregnant women were significantly higher as compared to normal healthy women. While serum total proteins (P<0.01), albumin (P<0.01) and total antioxidant status (TAS) (P<0.001) decreased significantly as compared to normal healthy women. Women in third trimester showed a significantly high level of body temperature (P<0.01), triglyceride (P<0.01), LDL-cholesterol (P<0.05), AST (P<0.01), T3 (P<0.01), homocysteine (P<0.001), TOS (P<0.01) and MDA (P<0.001) but a lower concentration of serum proteins, albumin and TAS at the end of the pregnancy. Pearson correlation indicated a positive relationship of homocysteine with triglycerides (P<0.027), TOS (P<0.01), MDA (P<0.035) and had a negative relationship with total protein (P<0.026). DNA damage was strongly related with T3 (P<0.008), TOS (P<0.02), MDA (P<0.037) and MBI (P<0.048) profiles of pregnant women. These changes were considered normal for pregnant women having optimum blood pressure and normal child birth. Hormonal influences and hemodilution may contribute towards the observed changes in this study.