1. In vivo imaging in an ABSL-3 regional biocontainment laboratory.
- Author
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Scanga CA, Lopresti BJ, Tomko J, Frye LJ, Coleman TM, Fillmore D, Carney JP, Lin PL, Flynn JL, Gardner CL, Sun C, Klimstra WB, Ryman KD, Reed DS, Fisher DJ, and Cole KS
- Subjects
- Animals, Disease Models, Animal, Pennsylvania, Technology, Radiologic methods, Bacterial Infections diagnosis, Bacterial Infections pathology, Containment of Biohazards, Optical Imaging methods, Virus Diseases diagnosis, Virus Diseases pathology
- Abstract
The Regional Biocontainment Laboratory (RBL) at the University of Pittsburgh is a state-of-the-art ABSL-3 facility that supports research on highly pathogenic viruses and bacteria. Recent advances in radiologic imaging provide several noninvasive, in vivo imaging modalities that can be used to longitudinally monitor animals following experimental infection or vaccination. The University of Pittsburgh RBL provides digital radiography, bioluminescence imaging, and PET/CT. Operating these platforms in an ABSL-3 poses unique challenges. This review will discuss the development and refinement of these imaging platforms in high containment, emphasizing specific challenges and how they were overcome., (© 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.)
- Published
- 2014
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