1. Comparative analysis of methanogen diversity in the rumen of crossbred buffalo and cattle in the Philippines by using the functional gene mcrA.
- Author
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Lwin KO, Matsui H, Ban-Tokuda T, Kondo M, Lapitan RM, Herrera JR, Del-Barrio AN, and Fujihara T
- Subjects
- Amino Acid Sequence, Animals, Breeding, Female, Genes, Bacterial genetics, Male, Molecular Sequence Data, Philippines, Phylogeny, Buffaloes microbiology, Cattle microbiology, Crosses, Genetic, Genetic Variation, Methanobacteriales genetics, Oxidoreductases genetics, Rumen microbiology
- Abstract
Comparative analyses of methanogen diversity in the rumen of crossbred buffalo and cattle fed the same diet in the Philippines was performed by cloning the methyl coenzyme M reductase A (mcrA) gene. The cattle and buffalo libraries consisted of 50 clones each. Comparative analysis of the amino acid sequence revealed that these 2 libraries differed significantly (P < 0.01). The deduced amino acid sequences of the clones were classified into 9 operational taxonomic units (OTUs) in buffalo and 11 OTUs in cattle. Sequence similarity between the clones and known cultured methanogens ranged from 86 to 97 % for buffalo and 84 to 99 % for cattle. Methanobrevibacter species were predominant in buffalo (64 % of the clones), and an unknown mcrA was predominant in cattle (52 % of the clones). A large number of clones with low similarity to cultivated methanogens was observed in both buffalo and cattle, suggesting the presence of an unknown methanogen species in their rumen.
- Published
- 2012
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