1. SRGAP1 is a candidate gene for papillary thyroid carcinoma susceptibility.
- Author
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He H, Bronisz A, Liyanarachchi S, Nagy R, Li W, Huang Y, Akagi K, Saji M, Kula D, Wojcicka A, Sebastian N, Wen B, Puch Z, Kalemba M, Stachlewska E, Czetwertynska M, Dlugosinska J, Dymecka K, Ploski R, Krawczyk M, Morrison PJ, Ringel MD, Kloos RT, Jazdzewski K, Symer DE, Vieland VJ, Ostrowski M, Jarząb B, and de la Chapelle A
- Subjects
- Carcinoma metabolism, Carcinoma, Papillary, Cell Line, Tumor, Cohort Studies, Enzyme Activation, Family Health, Female, GTPase-Activating Proteins chemistry, GTPase-Activating Proteins metabolism, Genome-Wide Association Study, HEK293 Cells, Humans, Linkage Disequilibrium, Male, Ohio, Pedigree, Poland, Protein Isoforms chemistry, Protein Isoforms genetics, Protein Isoforms metabolism, Protein Structure, Tertiary, Recombinant Proteins chemistry, Recombinant Proteins metabolism, Thyroid Cancer, Papillary, Thyroid Neoplasms metabolism, cdc42 GTP-Binding Protein genetics, cdc42 GTP-Binding Protein metabolism, Carcinoma genetics, GTPase-Activating Proteins genetics, Genetic Predisposition to Disease, Mutation, Missense, Thyroid Neoplasms genetics
- Abstract
Background: Papillary thyroid carcinoma (PTC) shows high heritability, yet efforts to find predisposing genes have been largely negative., Objectives: The objective of this study was to identify susceptibility genes for PTC., Methods: A genome-wide linkage analysis was performed in 38 families. Targeted association study and screening were performed in 2 large cohorts of PTC patients and controls. Candidate DNA variants were tested in functional studies., Results: Linkage analysis and association studies identified the Slit-Robo Rho GTPase activating protein 1 gene (SRGAP1) in the linkage peak as a candidate gene. Two missense variants, Q149H and A275T, localized in the Fes/CIP4 homology domain segregated with the disease in 1 family each. One missense variant, R617C, located in the RhoGAP domain occurred in 1 family. Biochemical assays demonstrated that the ability to inactivate CDC42, a key function of SRGAP1, was severely impaired by the Q149H and R617C variants., Conclusions: Our findings suggest that SRGAP1 is a candidate gene in PTC susceptibility. SRGAP1 is likely a low-penetrant gene, possibly of a modifier type.
- Published
- 2013
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