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The aim of this study was to determine the occurrence of urogenital mycoplasmas in urogenital tract of women with systemic lupus erythematosus (SLE). The study group included 36 women diagnosed with SLE and 100 healthy controls. Mycoplasmas were detected with microculture and PCR in 13/36 (36.1%) women with SLE and in 25/100 (25%) controls. In both groups, U. parvum occurred significantly more frequently. M. genitalium was detected in 3/36 (8.3%) SLE patients and in 3/100 (3%) controls. FVU as a material decreased the number of positive results from 33.3% to 30.6% compared with swabs.Although the incidence of mycoplasmas in urogenital tract of women with SLE and controls did not differ statistically, it is important to consider them as a potential etiology of urogenital infection when clinical symptoms are present, but etiology is unknown or uncertain.

Objectives: Preterm premature rupture of membranes (PPROM) complicates about 5% of pregnancies. Ureaplasma species is the most common pathogen found in the amniotic fluid in pregnancieneonatal outcome. The aim of the following study was to evaluate the impact of colonization with the Ureaplasma spp. on pregnant women with PPROM, coin fection with different microorganisms, and antimicrobial treatment on neonatal outcome., Material and Methods: The study included 30 women with PPROM hospitalized in Division of Reproduction in s complicated by PPROM. It is speculated that it requires a coin fection to produce unfavorable Poznan's K. Marcinkowski University of Medical Sciences. Swabs from cenvical canal were obtained for the identifidation of bacterial and ureaplasma tic infections by culture and POR., Results: The presence of any infection during the pregnancy a fter PP ROM was con firmed in 22 patients (Ureaplasma spp. in 12 patients, coin fection in 10 women). The cure rate for Ureaplasma species and other infections was 17% (2/12 patients) and 23% (5/22 patients), respectively There was no correlation between Ureaplasma species infection, coin fection, and cure status with the infection in the newborn. The PPROM to delivery duration also did not affect the newborn infection status. A negative relationship with leukocyte level was detected in patient with newborn infection., Conclusions: The presence of colonization with Ureaplasma species is not attributable to neonatal short-term morbidity The evaluation of maternal biochemical and microbiological data, regardless of the duration of the pregnancy after PPROM or the cure status, does not add any insight into the newborn infection status.

Objectives: The study was performed to evaluate the frequency of cervical infections with Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis in women with normal pregnancy and with symptoms of spontaneous abortion or preterm delivery. Antioxidant status of pregnant women was assessed. Elevated oxidative status is claimed to be one of the factors causing threatened miscarriage and premature labor., Material and Methods: From 73 women endocervical culture was investigated for Chlamydia trachomatis (method of rRNA Hybridization), Ureaplasma urealyticum and Mycoplasma hominis (Mycoplasma IST 2 test). Gestational age ranged from 14-30 weeks. They were categorized into 2 groups: patients not presenting these symptoms without history of pregnancy complications and patients with threatened abortion or symptoms of preterm delivery Following oxidative status factors were measures: Superoxide Dysmutase, Catalase and Glutathion Peroxidase in blood and saliva of 49 pregnant women of both groups were elevated. Statistica: the Student's T test, chi-square test, U Manna-Whithney's test., Results: The results show difficulty in assessment of direct influence of Chlamydia infection on oxidative stress. Ch. trachomatis was present in 9.6%, M. hominis in 6.8%, U. urealyticum in 28.8% of vaginal cultures. Chlamydiasis statistically increases production of Reactive Oxygen Forms what results in unfavorable outcome of the pregnancy Increase of the following parameters was observed: Superoxide Dysmutase (5583.2 vs 8980.2U/ml/sec) and Catalase (15.1 vs 18.3U/ml/sec)., Conclusion: Infection of Chlamydia trachomatis could be a significant factor in pathogenesis of threatened abortion and preterm delivery as it increases oxidative stress.

Unlabelled: Pregnancy promotes ureaplasma vaginal colonization. This creates the possibility of vertical transmission of these organisms to the child. These microorganisms can cause complications during pregnancy and poor condition of newborn., Objectives: Objectives of this study were to analyze the vertical transmission of different species of ureaplasmas in term newborns without respiratory distress., Materials and Methods: The study included 50 mothers and 50 of their newborn children. Swabs were obtained from swabs of the cervix in women and tracheal aspirates from neonates. The presence of ureaplasmas was confirmed by culture and PCR. Ureaplasmas species identification was performed using PCR., Results: infection of ureaplasmas was found in 21 women (42%). Predominant species was U. parvum, which was found in 18 women. In 3 patients only the presence of U. urealyticum was confirmed. Ureaplasma infection in mother and her newborn baby was confirmed in 8 (17.4%) mother-child pairs, including 6 of these cases showing the presence of U. parvum and 2 U. urealyticum. The incidence of vertical transmission of ureaplasma infection was assessed at 33% for U. parvum and 67% for U. urealyticum, and the total for both species at 38%. It should be noted that in the group of 18 women infected with U.parvum, in 12 cases there was no transmission of infection to the child. However in 3 women infected with U. urealyticum 2 cases of transmission from mother to child were observed (67%). Although the group infected with U. urealyticum accounted for only 3 women, our preliminary observations may suggest that this species is probably more likely to be transferred from mother to child., Conclusions: Infection with U. urealyticum may be more frequently transferred from the genital tract of mother to child.

The aim of this study was to evaluate the occurrence of genital mycoplasmas, especially Ureaplasma parvum and Ureaplasma urealyticum, in women with atypical squamous cells of undetermined significance (ASCUS), low grade squamous intraepithelial lesions (LSIL) and high grade squamous intraepithelial lesions (HSIL), compared to women with normal cytology living in Katowice, Poland. Two sterile swabs were used to obtain material from the posterior vaginal fornix of 143 women with squamous intraepithelial lesions and 39 healthy women: first for general bacteriology, second for detection of urogenital mycoplasmas using Mycoplasma IST2 kit. From each positive Mycoplasma IST2 culture DNA was isolated and PCR was performed for identification of U. parvum and U. urealyticum. Mycoplasma IST was positive in 34.1% cases. Urogenital mycoplasmas were demonstrated in women with HSIL significantly more often compared to women with LSIL, ASCUS, and with normal cytology. DNA of U. parvum was demonstrated in majority of Mycoplasma IST2-positive cases, U. urealyticum DNA-only in 9 (4.9%). Predominance of 3/14 serovars of U. parvum was demonstrated. U. urealyticum biovar 2 was present more often in women with squamous intraepithelial lesions.

The group of organisms commonly referred to as genital mycoplasmas comprise species most often found in genitourinary tract of sexually active adults as common commensal inhabitants, or pathogens which can possibly cause many different pathologies like: non-gonococcal urethritis, bacterial vaginosis, cervicitis, endometritis or pelvic inflammatory disease. The problem of their morbidity and the possible influence they have on human fertility is still not clear. The aim of this study was to find out whether two investigated species- Ureaplasma urealyticum and Mycoplasma hominis can be detect more often in a group of infertile women. 74 women participated in the study and were assigned to one of 2 groups of patients: infertile women and fertile women without any sign of genital tract infection. Swabs from the cervical canal of the uterus and the fluid from the Douglas pouch were taken during the gynecological examination and laparoscopic procedure. Two diagnostic methods were used: biochemical method- commercial diagnostic kit- Mycoplasma IST 2 and PCR method. The results showed that Ureaplasma urealyticum and Mycoplasma hominis were detected among both fertile and infertile women with nearly the same frequency, much more often in cervical canal than in the Douglas pouch. Ureaplasma urealyticum was more common pathogen than Mycoplasma hominis in both groups and locations. The achieved results point out that the role of genital mycoplasmas in human infertility is still unclear and require further investigations.

Purpose: The aim of the study was to assess the incidence of Ureaplasma urealyticum (U. urealyticum) and Mycoplasma hominis (M. hominis) infection in women with urogenital diseases., Material and Methods: M. hominis and U. urealyticum was assessed in 541 women from gynaecological and STD outpatient clinics, aged 18-55 years. A Mycoplasma IST 2 kit was used for biochemical determination of mycoplasmal infections (BioMerieux). Additionally, 248 of patients were examined for Chlamydia trachomatis (C. trachomatis), Trichomonas vaginalis (T. vaginalis) and Candida albicans (C. albicans) infection. C. trachomatis was detected by direct immunofluorescence method. The standard culture methods (Biomed) were applied to detect T. vaginalis and C. albicans., Results: U. urealyticum was detected in 161 (29.8%), and M. hominis in 20 (3.7%) women. U. urealyticum infection alone was observed in 37/79 (46.8%), and 1/8 (12.5%) patient had only M. hominis infection. The U. urealyticum infection showed most frequent coexistence with C. albicans (29.1%), and less frequent with C. trachomatis (13.9%) and M. hominis infection (10.1%). The highest percentage of mycoplasma-positive cultures was found in patients of STD clinic and in infertile women. In patients with ureaplasmal infection only the most common clinical symptom was vaginal discharge and vulval/vaginal irritation. In 8.1% of the women, the course of U urealyticum infection was asymptomatic., Conclusions: The incidence rate of genitourinary infections due to U urealyticum was significantly higher as compared to M. hominis infection. Sexual mycoplasmal infections were most frequently reported in the group of patients of STD clinic and correlated with age and sexual activity.

Purpose: The aim of this study was to estimate the incidence of M. hominis and U. urealyticum infections among men with urethritis and its complications., Material and Methods: Material for analysis were urethral swabs and EPS. Mycoplasma IST 2 kit was applied to diagnose mycoplasmal infections. All patients were additionaly tested for C. trachomatis, C. albicans and T. vaginalis and Gram stain specimens were obtained to identify the presence of PMN., Results: U. urealyticum was detected in 57/390 (14.6%), and M. hominis in 4/390 (1%) men. Exclusive U. urealyticum infection was found in 45 (11.5%) men, and only 2 patients had exclusive M. hominis infection. U. urealyticum infection the most frequently coexisted with C. trachomatis--5 (8.8%), next with C. albicans--4 (7%) and M. hominis--2 (3.5%) infections. Mycoplasmal infections were the most frequently found in patients aged 30 to 39 (35.1%) diagnosed with epididymitis (29.2%). The most commonly reported symptom was dysuria., Conclusions: U. urealyticum is the common pathogen among men with urethritis and its complications. The most common symptoms in U urealyticum patients were: dysuria, hypogastric pains and urethrorrhoea, however, clinical symptoms are not frequently observed.

The retrospective analysis of group of 164 men from sterile couples was made. Authors compared spermiograms with bacteriological investigations' results (sperm bacteriology and blots from urethra for present of Chlamydia, Mycoplasma and Ureaplasma). The statistical signification of coincidence of leucospermia and one-species bacterial infection and dependence between leucospermia and pathological spermiogram were found. The positive results of sperm bacteriological investigations were found in normospermic patients too.