1. Characterization and genome sequence of the genetically unique Escherichia bacteriophage vB_EcoM_IME392.
- Author
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Hu, Yunjia, Tong, Shanwei, Li, Ping, An, Xiaoping, Song, Lihua, Fan, Huahao, and Tong, Yigang
- Subjects
BACTERIOPHAGES ,NUCLEOTIDE sequencing ,POULTRY farms ,ESCHERICHIA ,SEWAGE irrigation ,TRANSMISSION electron microscopy - Abstract
In this study, a novel Escherichia coli-specific bacteriophage, vB_EcoM_IME392, was isolated from chicken farm sewage in Qingdao, China. The genome of IME392 was found by next-generation sequencing to be 116,460 base pairs in length with a G+C content of 45.4% (GenBank accession number MH719082). BLASTn results revealed that only 2% of the genome sequence of IME392 shows sequence similarity to known phage sequences in the GenBank database, which indicates that IME392 is a novel bacteriophage. Transmission electron microscopy showed that IME392 belongs to the family Myoviridae. The host range, the multiplicity of infection, and a one-step growth curve were also determined. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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