Search

A strictly aerobic, Gram-stain-positive actinobacterial strain was isolated from a rhizosphere soil of a wild plant (Peucedanum japonicum Thumb.) collected on Mara Island, Jeju, Republic of Korea. Cells of strain RS-50(T) were oxidase-negative, catalase-positive, short rods and motile by means of a polar flagellum; the colonies were orange, circular, smooth and convex. meso-Diaminopimelic acid and glucose were the diagnostic diamino acid in the cell wall and the whole-cell sugar, respectively. The major menaquinone was MK-9(H(4)). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, two unknown phospholipids and an unknown lipid. The major fatty acids were iso-C(15 : 0), iso-C(16 : 0,) anteiso-C(15 : 0) and anteiso-C(17 : 0). The DNA G+C content was 73.6 mol%. In 16S rRNA gene sequence-based phylogenetic trees, strain RS-50(T) formed a tight cluster with Angustibacter luteus (99.2 % sequence similarity); both were loosely related to the suborders Kineosporiineae and Micrococcineae. The DNA-DNA relatedness value of the isolate to A. luteus KACC 14249(T) was 22.3±0.9 %. On the basis of the results of phenotypic analyses and DNA-DNA hybridization experiments, strain RS-50(T) is considered to represent a novel species of the genus Angustibacter, for which the name Angustibacter peucedani sp. nov. is proposed. The type strain is RS-50(T) ( = KCTC 19628(T) = DSM 45329(T)). The description of the genus Angustibacter is emended.

A novel actinomycete, designated strain RS-49(T), was isolated from the rhizosphere soil of a cliff-associated plant (Peucedanum japonicum Thunb.) in the Republic of Korea and subjected to a polyphasic taxonomic study. The results of comparative 16S rRNA gene sequence analyses showed that the organism belonged to the family Pseudonocardiaceae, suborder Pseudonocardineae and that it was most closely related to members of the genera Kibdelosporangium (96.6-97.0% sequence similarity), Actinokineospora (96.3-96.7%), Streptoalloteichus (96.2%) and Actinophytocola (96.2%). Substrate mycelia were well-developed and whitish or pale yellow to strong yellow. Aerial mycelia were branched and fragmented into rod-shaped elements. Single spherical spores were produced directly on the substrate mycelium. Sporangium-like structures and fragmentation of the substrate mycelium were absent. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The acyl type of the muramic acid residues in the peptidoglycan was N-acetylated. Whole-cell sugars were glucose, rhamnose, galactose, ribose, mannose, arabinose and xylose. The major menaquinone was MK-9(H(4)). Small amounts of MK-8 and MK-9(H(2)) were also detected. The polar lipids contained diphosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and an unknown lipid. The predominant fatty acids were iso-C(15:0) and iso-C(16:0). The DNA G+C content was 64.2 mol%. The phenotypic and phylogenetic characteristics show that strain RS-49(T) can be differentiated from members of all genera in the suborder Pseudonocardineae and thus represents a novel species in a new genus for which the name Labedaea rhizosphaerae gen. nov., sp. nov. is proposed; the type strain of the type species is RS-49(T) ( = KCTC 19662(T) = DSM 45361(T)).

A novel actinomycete strain, designated RP-AC37(T), was isolated from rhizosphere soil collected on Mara Island of Jeju, Republic of Korea. Cells were aerobic, gram-positive, oxidase-negative, catalase-positive, non-mycelium-forming and motile rods (0.6-0.7×1.9-2.4 µm). Phylogenetic analysis based on 16S rRNA gene sequences showed that the organism formed a distinct clade within the radiation occupied by the suborder Frankineae. 16S rRNA gene similarity values were less than 93.2 % to members of the suborder Frankineae and related taxa. The diamino acid isomer in the cell-wall peptidoglycan was LL-diaminopimelic acid. The major whole-cell sugars were glucose, galactose and xylose. The major menaquinone was MK-9(H(4)). The polar lipids were diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. The cellular fatty acids were straight-chain, unsaturated and saturated, with a significant amount of tuberculostearic acid (10-methyl-C(18 : 0)). The DNA G+C content was 73.2 mol%. The combination of morphological, chemotaxonomic and phylogenetic data clearly separate the isolate from members of known genera of the suborder Frankineae and related taxa, suggesting that the isolate represents a novel species in a new genus in this suborder, for which the name Motilibacter peucedani gen. nov., sp. nov. is proposed; the type strain is RP-AC37(T) ( = KCTC 19630(T) = DSM 45328(T)).