1. Determination and quantification of γ-glutamyl-valyl-glycine in commercial fish sauces.
- Author
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Kuroda M, Kato Y, Yamazaki J, Kai Y, Mizukoshi T, Miyano H, and Eto Y
- Subjects
- Animals, China, Chromatography, High Pressure Liquid, Fishes, Italy, Japan, Receptors, Calcium-Sensing, Republic of Korea, Tandem Mass Spectrometry, Taste, Thailand, Vietnam, Fish Products analysis, Oligopeptides analysis
- Abstract
It was recently reported that kokumi substances such as glutathione are perceived through the calcium-sensing receptor (CaSR). In addition, screening by the CaSR assay and sensory evaluation revealed that γ-glutamyl-valyl-glycine (γ-Glu-Val-Gly) was a potent kokumi peptide. In this study, the quantities of γ-Glu-Val-Gly in various commercial fish sauces originating from Vietnam (Nuoc Mum), Thailand (Nampra), China (Yu-lu), Korea, Japan (Shottsuru and Ikanago-shoyu), and Italy (Garum) were investigated using a LC/MS/MS method followed by derivatization with 6-aminoquinoyl-N-hydroxysuccinimidyl-carbamate (AQC). The analyses revealed γ-Glu-Val-Gly at concentrations ranging from 0.04 to 1.26 mg/dL, indicating that γ-Glu-Val-Gly is widely distributed among various commercial fish sauces.
- Published
- 2012
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