1. Melanogenesis inhibitory and fibroblast proliferation accelerating effects of noroleanane- and oleanane-type triterpene oligoglycosides from the flower buds of Camellia japonica.
- Author
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Nakamura S, Moriura T, Park S, Fujimoto K, Matsumoto T, Ohta T, Matsuda H, and Yoshikawa M
- Subjects
- Animals, Flowers drug effects, Humans, Melanins biosynthesis, Mice, Molecular Structure, Nuclear Magnetic Resonance, Biomolecular, Oleanolic Acid chemistry, Republic of Korea, Saponins chemistry, Skin cytology, Camellia chemistry, Fibroblasts drug effects, Melanoma, Experimental drug therapy, Oleanolic Acid isolation & purification, Oleanolic Acid pharmacology, Saponins isolation & purification, Saponins pharmacology
- Abstract
A 28-noroleanane-type triterpene oligoglycoside, camellioside E (4), an oleanane-type triterpene oligoglycoside, camellioside F (5), and the known compounds camelliosides A (1) and D (3) were isolated from a 50% EtOH extract of Camellia japonica flower buds from Korea. The principal constituents (1 and 5) significantly inhibited melanogenesis in theophylline-stimulated B16 melanoma 4A5 cells. Camellioside B (2), a major constituent of C. japonica grown in Japan, showed potent inhibition of melanogenesis [95.0 ± 1.0% (p < 0.01) at 20 μM]. The inhibitory effects of 1, 2, and 5 were stronger than that of the reference compound, arbutin. We believe the melanogenesis inhibitory effects of 2 and 5 are partly related to the proliferation inhibitory effects in B16 melanoma 4A5 cells. Conversely, camelliosides tended to enhance proliferation in normal human neonatal skin fibroblasts. Interestingly, camellioside B (2) significantly accelerated fibroblast proliferation. This biological selectivity could make camellioside B useful for treating skin disorders. Herein, we report the first scientific investigation of a triterpene that displays an inhibitory effect on melanogenesis, but that also has an enhancing effect on fibroblast proliferation.
- Published
- 2012
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