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Your search keyword '"Phloroglucinol isolation & purification"' showing total 2 results
Start Over Descriptor "Phloroglucinol isolation & purification" Region republic of korea
2 results on '"Phloroglucinol isolation & purification"'

1. First evidence that Ecklonia cava-derived dieckol attenuates MCF-7 human breast carcinoma cell migration.

Author

Kim EK, Tang Y, Kim YS, Hwang JW, Choi EJ, Lee JH, Lee SH, Jeon YJ, and Park PJ

Subjects
Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, Benzofurans chemistry, Benzofurans pharmacology, Breast Neoplasms metabolism, Breast Neoplasms pathology, Cell Movement drug effects, Cell Survival drug effects, Dioxanes chemistry, Dioxanes isolation & purification, Dioxanes pharmacology, Dioxins chemistry, Dioxins isolation & purification, Female, Gene Expression Regulation, Neoplastic drug effects, Humans, MCF-7 Cells, Matrix Metalloproteinase 9 chemistry, Matrix Metalloproteinase 9 genetics, Matrix Metalloproteinase 9 metabolism, Neoplasm Proteins agonists, Neoplasm Proteins antagonists & inhibitors, Neoplasm Proteins genetics, Neoplasm Proteins metabolism, Pacific Ocean, Phaeophyceae growth & development, Phloroglucinol analogs & derivatives, Phloroglucinol chemistry, Phloroglucinol isolation & purification, Phloroglucinol pharmacology, Republic of Korea, Seaweed growth & development, Tannins chemistry, Tannins pharmacology, Tissue Inhibitor of Metalloproteinase-1 agonists, Tissue Inhibitor of Metalloproteinase-1 genetics, Tissue Inhibitor of Metalloproteinase-1 metabolism, Tissue Inhibitor of Metalloproteinase-2 agonists, Tissue Inhibitor of Metalloproteinase-2 genetics, Tissue Inhibitor of Metalloproteinase-2 metabolism, Vascular Endothelial Growth Factor A antagonists & inhibitors, Vascular Endothelial Growth Factor A genetics, Vascular Endothelial Growth Factor A metabolism, Antineoplastic Agents isolation & purification, Benzofurans isolation & purification, Breast Neoplasms drug therapy, Drug Discovery, Phaeophyceae chemistry, Seaweed chemistry, Tannins isolation & purification
Abstract

We investigated the effect of Ecklonia cava (E. cava)-derived dieckol on movement behavior and the expression of migration-related genes in MCF-7 human breast cancer cell. Phlorotannins (e.g., dieckol, 6,6'-biecko, and 2,7″-phloroglucinol-6,6'-bieckol) were purified from E. cava by using centrifugal partition chromatography. Among the phlorotannins, we found that dieckol inhibited breast cancer cell the most and was selected for further study. Radius™-well was used to assess cell migration, and dieckol (1-100 µM) was found to suppress breast cancer cell movement. Metastasis-related gene expressions were evaluated by RT-PCR and Western blot analysis. In addition, dieckol inhibited the expression of migration-related genes such as matrix metalloproteinase (MMP)-9 and vascular endothelial growth factor (VEGF). On the other hand, it stimulated the expression of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2. These results suggest that dieckol exerts anti-breast cancer activity via the regulation of the expressions of metastasis-related genes, and this is the first report on the anti-breast cancer effect of dieckol.

Published
2015
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2. Anti-inflammatory activity of edible brown alga Eisenia bicyclis and its constituents fucosterol and phlorotannins in LPS-stimulated RAW264.7 macrophages.

Author

Jung HA, Jin SE, Ahn BR, Lee CM, and Choi JS

Subjects
Animals, Anti-Inflammatory Agents analysis, Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents isolation & purification, Cell Line, Transformed, Cell Survival drug effects, Cyclooxygenase 2 chemistry, Cyclooxygenase 2 metabolism, Dioxanes adverse effects, Dioxanes analysis, Dioxanes isolation & purification, Down-Regulation drug effects, Macrophages enzymology, Macrophages immunology, Macrophages metabolism, Mice, Nitric Oxide antagonists & inhibitors, Nitric Oxide metabolism, Nitric Oxide Synthase Type II antagonists & inhibitors, Nitric Oxide Synthase Type II metabolism, Osmolar Concentration, Oxidative Stress drug effects, Phloroglucinol adverse effects, Phloroglucinol analysis, Phloroglucinol isolation & purification, Phloroglucinol pharmacology, Plant Extracts adverse effects, Plant Extracts chemistry, Plant Extracts isolation & purification, Plant Leaves chemistry, Reactive Oxygen Species antagonists & inhibitors, Reactive Oxygen Species metabolism, Republic of Korea, Solvents chemistry, Stigmasterol adverse effects, Stigmasterol analysis, Stigmasterol isolation & purification, Stigmasterol pharmacology, Anti-Inflammatory Agents metabolism, Dioxanes pharmacology, Functional Food analysis, Kelp chemistry, Macrophages drug effects, Phloroglucinol analogs & derivatives, Plant Extracts pharmacology, Stigmasterol analogs & derivatives
Abstract

Although individual phlorotannins contained in the edible brown algae have been reported to possess strong anti-inflammatory activity, the responsible components of Eisenia bicyclis have yet to be fully studied. Thus, we evaluated their anti-inflammatory activity via inhibition against production of lipopolysaccharide (LPS)-induced nitric oxide (NO) and tert-butylhydroperoxide (t-BHP)-induced reactive oxygen species (ROS), along with suppression against expression of inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), in RAW 264.7 cells. The anti-inflammatory activity potential of the methanolic extract and its fractions of E. bicyclis was in the order of dichloromethane>methanol>ethyl acetate>n-butanol. The strong anti-inflammatory dichloromethane fraction was further purified to yield fucosterol. From the ethyl acetate fraction, six known phlorotannins were isolated: phloroglucinol, eckol, dieckol, 7-phloroeckol, phlorofucofuroeckol A and dioxinodehydroeckol. We found that these compounds, at non-toxic concentrations, dose-dependently inhibited LPS-induced NO production. Fucosterol also inhibited t-BHP-induced ROS generation and suppressed the expression of iNOS and COX-2. These results indicate that E. bicyclis and its constituents exhibited anti-inflammatory activity which might attribute to inhibition of NO and ROS generation and suppression of the NF-κB pathway and can therefore be considered as a useful therapeutic and preventive approach to various inflammatory and oxidative stress-related diseases., (Copyright © 2013 Elsevier Ltd. All rights reserved.)

Published
2013
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