1. Structure Elucidation and in Vitro Toxicity of New Azaspiracids Isolated from the Marine Dinoflagellate Azadinium poporum.
- Author
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Krock B, Tillmann U, Potvin É, Jeong HJ, Drebing W, Kilcoyne J, Al-Jorani A, Twiner MJ, Göthel Q, and Köck M
- Subjects
- Humans, Jurkat Cells, Leukemia, T-Cell metabolism, Magnetic Resonance Spectroscopy, Marine Toxins chemistry, Marine Toxins toxicity, Republic of Korea, Species Specificity, Spiro Compounds chemistry, Spiro Compounds toxicity, Toxicity Tests, Dinoflagellida metabolism, Marine Toxins isolation & purification, Spiro Compounds isolation & purification
- Abstract
Two strains of Azadinium poporum, one from the Korean West coast and the other from the North Sea, were mass cultured for isolation of new azaspiracids. Approximately 0.9 mg of pure AZA-36 (1) and 1.3 mg of pure AZA-37 (2) were isolated from the Korean (870 L) and North Sea (120 L) strains, respectively. The structures were determined to be 3-hydroxy-8-methyl-39-demethyl-azaspiracid-1 (1) and 3-hydroxy-7,8-dihydro-39-demethyl-azaspiracid-1 (2) by ¹H- and (13)C-NMR. Using the Jurkat T lymphocyte cell toxicity assay, (1) and (2) were found to be 6- and 3-fold less toxic than AZA-1, respectively.
- Published
- 2015
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