1. Haloferax prahovense sp. nov., an extremely halophilic archaeon isolated from a Romanian salt lake.
- Author
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Enache M, Itoh T, Kamekura M, Teodosiu G, and Dumitru L
- Subjects
- Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, DNA, Archaeal chemistry, DNA, Archaeal isolation & purification, DNA, Ribosomal chemistry, DNA, Ribosomal isolation & purification, Genes, rRNA, Haloferax growth & development, Hydrogen-Ion Concentration, Lipids analysis, Magnesium Chloride, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Archaeal genetics, RNA, Ribosomal, 16S genetics, Romania, Sequence Analysis, DNA, Sodium Chloride, Temperature, Haloferax classification, Haloferax isolation & purification, Water Microbiology
- Abstract
A novel halophilic archaeon, strain TL6T, was isolated from Telega Lake, a hypersaline environment in Prahova county, Romania. Strain TL6T was able to grow in media with a salt concentration of between 2.5 and 5.2 M, with optimum growth at a concentration of 3.5 M. The novel strain was able to grow at concentrations of 1 M MgCl2 or less, with an optimum of 0.4 M Mg2+. Growth of the novel strain occurred between pH 6.0 and 8.5, with an optimum of pH 7.0-7.5. The G+C content of the total DNA was 63.7 mol%. The 16S rRNA gene sequence of the novel strain was most closely related to species of the genus Haloferax (97.3-99.3 % sequence similarity). The lipid profile of the novel strain corresponded to that of other species belonging to the genus Haloferax. A comparative analysis of the phenotypic properties and DNA-DNA hybridization between the novel strain and other species of the genus Haloferax strongly supported the conclusion that strain TL6T represents a novel species within this genus, for which the name Haloferax prahovense sp. nov., is proposed. The type strain is TL6T (=JCM 13924T=DSM 18310T).
- Published
- 2007
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