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The paper presents the study of the soil quality and health expressed by the chemical and biological properties in a research field placed at Varciorog, Bihor County, Romania. The soil samples were collected from 3 soil variants in March 2023. In each soil variant, some soil chemical parameters and the abundance of bacteria were determined. The frequency and intensity of colonization, along with arbuscules and vesicles, were scored to determine the mycorrhizal potential of each soil. The community-level physiological profile was used to determine the functional microbiome and its ability to decompose a specific set of substrates. In the control variant (CTRL), which is a functional forest cultivated with beech in a proportion of 90%, the soil properties were compared with those determined from Site 1 (a declined mixed forest) and from Site 2 (chestnut forest in a stage of complete drying). The data were statistically processed with a one-way ANOVA test, followed by the Duncan post-hoc test, which revealed significant variation in the potential of microbial functional communities across the analyzed sites. Also, the soil parameters that significantly varied in the 3 soil variants were bacterial number, pH, humus, exchangeable aluminum, coarse sand, dust, and fine sand. The Pearson correlation was computed to study the links between bacterial numbers and chemical parameters. The results showed strong correlations between most of the studied soil properties. The Ecoplates approach to soil functional microbiome highlighted various differences between the microbial communities of the three tested sites. Mycorrhizal colonization shows different potentials for symbiosis formation. The peak of mycorrhizal colonization was in declined forest, with 43.36% colonization frequency and 24.56% intensity. Arbuscules reached 11.36% in declined forest, while in control and decayed sites, the indicator was under 4%. Vesicles are more associated with control and decayed forests, with values of presence over 1.30%. As an indicator of microbial general activity, the sum of recorded activities was higher in declined and decayed forests. At these sites, the activity of the functional microbiome was amplified. The decline process activates a higher diversity of functional groups and is associated with a larger area of substrate decomposition capacity, which indicates a more extensive range of microbial functions related to breaking down organic matter. [ABSTRACT FROM AUTHOR]

Medicinal and aromatic plants hold a high share of interest in Romania. To offset the harvesting of spontaneous flora and ensure the sustainable conservation of natural resources, the cropping of highly valuable plants of interest represents a priority for the current agricultural system. This study was conducted due to the need for more balance in species exploitation. Therefore, it assessed the emergence dynamics, leaf appearance, growth and development of the plants depending on row spacing and plant distance. The research was conducted at the INCDCSZ Brașov, within the Technology Department, Laboratory of Medicinal and Aromatic Plants. The density of planting is the most important factor. Plants sown in continuous rows showed the highest yields of fresh and dry herbs, especially when the distance between the rows was set to 25 cm. Row-by-row distances of 50 cm ensure average values for all growth and development parameters. An increase in the row-by-row distance up to 70 cm drastically reduced the dry biomass up to 7.89 t ha−1 in continuous rows, followed by a 2 and 2.5 t ha−1 decrease for 15 cm and 25 cm plant-by-plant distances, respectively. Continuous row planting and a 25 cm row-by-row distance negligibly reduce the climate impact on growth and development. Greater spaces between plants leads to an average increase in individual development, but with a decrease in the total potential yield. [ABSTRACT FROM AUTHOR]

By studying mycorrhizal symbiosis, another important step is made in knowing the relationship between plants and soil microflora. Benefits that these symbiosis bring to plants and ecosystem are semnificative for the interpretation of physiological factors, like: extending the volume of soil accessible to plants, acquiring nutrient forms that would not normally be available to plants; for the ecosystem an important role is to soil structure and mechanical aggregation. In the pasture that we studied in the Apuseni Mountains we found as dominant species Carex hirta, percent in the floristic composition is 35-40%, and that is the primary reason for studying this plant. The study area is positioned at 2 meters far from the forest and the soil is caracterized by a big humidity - the subteran water is at a high level. To identify the influence of the forest, of the humidity and the relation with other plants in the pasture, first step was to find that plants of Carex hirta is colonized by mycorrhizal symbiosis. We harvest the plants with root from a 15 to 20 cm deep and we colored teh roots with a solution of ink with vinegar. The proposed method for diagnosis of mycorrhizal colonization on Carex hirta roots is specific for the area where the studies took place. [ABSTRACT FROM AUTHOR]

Regional climate projections are widely used in impact studies such as adaptations in agronomy. The big challenge of the climate modeling community is to serve valuable instructions regarding the reliability of these simulations to encourage agronomists to use this kind of information properly. The study validates 15 high-resolution ensembles from the Coordinated Regional Climate Downscaling Experiment-European Domain (EURO-CORDEX) for maximum temperature, minimum temperature, and precipitation to fulfill this task. Three evaluation metrics are calculated (mean absolute error, root mean square error, and correlation) for the means and the 5th and 95th percentiles. The analyses are elaborated for annual and monthly means and the vegetation periods of maize and winter wheat. Only arable lands are considered to exclude the effects of the topography. Furthermore, an ensemble selection is applied based on the evaluation metrics to reduce the data use. The five models with the best performance in the case of winter wheat are CNRM-CM5-CLMcom-CCLM4-8-17_v1, MOHC-HadGEM2-ES-IPSL-WRF381P_v1, MOHC-HadGEM2-ES-KNMI-RACMO22E_v2, MOHC-HadGEM2-ES-CLMcom-CCLM4-8-17_v1, and MPI-M-MPI-ESM-LR-KNMI-RACMO22E_v1. In the case of the vegetation period of maize, the models with the best skills are MPI-M-MPI-ESM-LR-KNMI-RACMO22E_v1, CNRM-CM5-IPSL-WRF381P_v2, MPI-M-MPI-ESM-LR-SMHI-RCA4_v1a, MOHC-HadGEM2-ES-IPSL-WRF381P_v1, and MOHC-HadGEM2-ES-KNMI-RACMO22E_v2. Quantifying the errors in climate simulations against observations and elaborating a selection procedure, we developed a consistent ensemble of high time and space resolution climate projections for agricultural use in Romania. [ABSTRACT FROM AUTHOR]

High nature value (HNV) grassland systems are increasingly important for the ecosystem services they provide and for their socio-economic impact in the current constant-changing context. The aim of our paper is to evaluate the long-term effect of organic fertilizers on HNV systems in the Apuseni Mountains, Romania. As an objective we want to identify the optimal intensity of conservation management and its recognition based on indicator value plant species. The experiments were established in 2001 on the boreal floor and analyze the effect of a gradient of four organic treatments with manure. Fertilization with 10 t ha−1 manure ensures an increase in yield and has a small influence on diversity, and could be a real possibility for the maintenance and sustainable use of HNV. Each fertilization treatment determined species with indicator value that are very useful in the identification and management of HNV. The indicator species are useful in forecasting the fluctuations and successions in grasslands, determined by the modification of the dominance-codominance ratio and the real coverage of each species. [ABSTRACT FROM AUTHOR]