Background: Surgical treatment of intramedullary spinal cord tumors is aimed at total resection of tumor with maximum preservation of neurological and functional status. In some cases, intramedullary tumors have unclear dissection plane or gliosis zone. This area is not a tumor and does not require resection. However, it is difficult to distinguish visually intact spinal cord tissue and tumor at the last surgical stages. Thus, we evaluated the effectiveness of fluorescence combined with laser spectroscopy in surgical treatment of intramedullary spinal cord tumors., Objective: To determine the effectiveness of visual fluorescence combined with laser spectroscopy in surgery for intramedullary spinal cord tumors., Material and Methods: There were 850 patients with intramedullary spinal cord tumors for the period 2001-2019. In 35 cases, intraoperative fluoroscopy with laser spectroscopy were used. All patients underwent a comprehensive pre- and postoperative clinical and instrumental examination (general and neurological status, McCormick grade, spinal cord MRI). Carl Zeiss OPMI Pentero microscope with a fluorescent module was used for intraoperative fluorescence diagnosis. A domestic preparation 5-ALA «ALASENS» (State Research Center NIOPIK, Moscow, Russia) was used for induction of visible fluorescence. Laser spectroscopy was carried out using a LESA-01-BIOSPEK spectrum analyzer. Morphological analysis of intramedullary spinal cord tumors was performed in the neuromorphology laboratory of the Burdenko Neurosurgery Center., Results: Intramedullary anaplastic ependymoma and astrocytoma, as well as conventional ependymoma were characterized by the highest index of 5-ALA accumulation. Intramedullary hemangioblastoma and cavernoma do not accumulate 5-aminolevulinic acid due to morphological structure of these tumors. In particular, there are no cells capable of capturing and processing 5-ALA in these tumors. Sensitivity of visual fluorescence combined with laser spectroscopy varies from 0% to 100% depending on the histological type of tumor: hemangiogblastoma and cavernoma - 0%, low-grade astrocytoma - 70%, high-grade astrocytoma - 80%, ependymoma - 92%, anaplastic ependymoma 100%. Dissection plane is absent in anaplastic ependymoma, high-grade astrocytoma. We often observed gliosis during resection of ependymoma. This tissue is not a part of tumor. Intraoperative metabolic navigation with neurophysiological monitoring are advisable for total tumor resection in case of unclear dissection plane and peritumoral gliosis., Conclusion: Visual fluorescence combined with laser spectroscopy is a perspective method for intraoperative imaging of tumor remnants and total resection of intramedullary spinal cord tumors with minimum risk of neurological impairment.