1. Genetic Basis of Attenuation of Cold-Adapted Influenza Strain B/Leningrad/14/17/55 — Backup Master Donor Virus for Influenza Type B Live Attenuated Vaccines.
- Author
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Krutikova, E. V., Stepanova, E. A., Wong, P. F., Kiseleva, I. V., Grigor'eva, E. P., and Rudenko, L. G.
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INFLUENZA B virus , *INFLUENZA vaccines , *VACCINES , *INFLUENZA , *INFLUENZA viruses - Abstract
The reassortant vaccine strain of live attenuated influenza vaccine inherits temperature sensitivity and areactogenicity from cold-adapted attenuated master donor virus. In Russia, B/ USSR/60/69 master donor virus (B60) is currently in use for the preparation of live attenuated type B influenza vaccine candidates. Trivalent live attenuated influenza vaccine based on A/ Leningrad/134/17/57 and B60 are licensed for the use in Russia for single dose vaccination of adults and children over 3 years. B/Leningrad/14/17/55 (B14) cold-adapted virus is a backup master donor virus for live attenuated type B influenza vaccine. According to our preliminary estimates, it is more attenuated than B60, which can allow expanding applicability of this vaccine for children under 3 years of age. In this paper, the role of B14 genes in its attenuation was assessed. Representative collection of reassortants of B14 with epidemic influenza B viruses was obtained, a phenotypic analysis of reassortants was performed, and their pathogenicity for animals was assessed. The leading role of PB2 and PA genes in attenuation of B14 master donor virus was proven. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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