1. Biochemical alterations in caged Nile tilapia Oreochromis niloticus
- Author
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Franco, Jeferson Luis, Trevisan, Rafael, Posser, Thais, Trivella, Daniela B.B., Hoppe, Roberto, Martins Rosa, Juliana, Fernandes Dinslaken, Daniel, Decker, Helena, Inês Tasca, Carla, Bainy Leal, Rodrigo, Freire Marques, Maria Risoleta, Dias Bainy, Afonso Celso, and Luiz Dafre, Alcir
- Subjects
NILE tilapia ,WATERSHED ecology ,OXIDATIVE stress -- Risk factors ,GLUTATHIONE transferase ,CATALASE ,STATISTICAL correlation ,TRANSFERASES ,PHYSIOLOGY - Abstract
Joinville is an important industrial city in Santa Catarina, southern Brazil, and also a risk factor for the Babitonga drainage basin. Oxidative stress-related parameters were evaluated in caged tilapia (Oreochromis niloticus) exposed for 7 days (sites S1 and S2) in a Babitonga drainage basin tributary river. Site S1 showed enhanced levels of hepatic CYP1A, CYP2B-like and glutathione S-transferase activity, while site S2 showed decreased levels of glutathione and increased lipoperoxidation indexes, catalase, glutathione peroxidase and glutathione reductase activity. Correlation analyses revealed that oxidative stress-related parameters behaved like a group of interrelated variables, while CYPs and glutathione S-transferase seem to be independent. New putative biomarkers were evaluated in the tilapia brain. Caspase-3 activation (both sites), decreased in p38MAPK phosphorylation (site S2) and decreased expression in HSP70 (site S1) were observed. Data indicate that employed variables, when used as a group (oxidative stress-related parameters, CYP1A/2B-like, caspase-3, HSP70 and protein kinases) can be useful as predictors of pollution. [Copyright &y& Elsevier]
- Published
- 2010
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