1. Detection of serum biomarkers of HPV-16 driven oropharynx and oral cavity cancer in Brazil.
- Author
-
Sichero, Laura, Gonçalves, Milena Giulia, Bettoni, Fabiana, Coser, Elisangela Monteiro, Mota, Giana, Nunes, Rafaella A.L., Mercante, Ana Maria da Cunha, Natalino, Renato, Uno, Miyuki, Ferreira Alves, Maria José, Matos, Leandro Luongo, Kowalski, Luiz Paulo, Kulcsar, Marco Aurélio Vamondes, de Alvarenga, Gustavo Fernandes, Höfler, Daniela, Schroeder, Lea, Waterboer, Tim, Tommasino, Massimo, and Villa, Luisa Lina
- Subjects
- *
ORAL cancer , *OROPHARYNX , *BIOMARKERS , *CIRCULATING tumor DNA , *HUMAN papillomavirus - Abstract
• We analyzed OPC and OCC samples obtained from individuals treated at ICESP, São Paulo, Brazil. • The prevalence of HPV-16 attributable cases was very low. None OCC cases was HPV-driven. • HPV-16 ctDNA was more commonly detected than HPV-16 E6 antibodies. • 100% sensitivity for detecting HPV-16 driven OPC was observed for both biomarkers, although the specificity of HPV-16 anti-E6 was higher. • Serum biomarkers are highly sensitive and specific biomarker for the detection of HPV-associated OPC. HPV-16 driven oropharynx/oral cavity squamous cell carcinomas prevalence varies globally. We evaluated the presence of HPV-16 ctDNA and HPV-16 E6 antibodies in samples obtained from participants treated at the Instituto do Cancer do Estado de Sao Paulo, ICESP, and from whom tumoral HPV DNA, HPV-16 E6*I mRNA, and p16INK4a status was also accessed. HPV was genotyped by PCR-hybridization. All HPV DNA positive and ∼10 % HPV DNA negative cases underwent p16INK4a immunohistochemistry and E6*I RNA testing using a multiplex bead based protocol. HPV-16 ctDNA and anti-E6 antibodies were assessed by ddPCR (digital droplet PCR) and multiplex serology, respectively. The prevalence of HPV-16 in oropharynx carcinoma (OPC) cases was low (8.7 %) when considering solely HPV-16 DNA detection, and even lower (5.2 %) when taken into consideration the concomitant detection of HPV-16 E6*I RNA and/or p16INK4 (HPV-16 attributable fraction - AF). None of the oral cavity cancer (OCC) cases were detected with HPV-16 DNA. HPV-16 ctDNA was more commonly detected than HPV-16 E6 antibodies (29.8 % versus 10.6 %). Both serum biomarkers attained 100 % sensitivity of detecting HPV-16 AF OPC, however the specificity of the HPV-16 anti-E6 biomarker was higher compared to ctDNA (93.2 % versus 75.0 %). Finally, when both HPV-16 ctDNA and anti-E6 biomarkers were considered together, the sensitivity and specificity for HPV-16 OPC detection was 100 % and about 70 %, respectively, independently of analyzing HPV-16 DNA positive or HPV-16 AF tumors. Our findings corroborate that serum biomarkers are highly sensitive and specific biomarkers for detection of HPV-associated OPC. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF