1. Occurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China.
- Author
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Dan, Jiaming, Zhang, Xueping, Ren, Zhihua, Wang, Liqin, Cao, Suizhong, Shen, Liuhong, Deng, Junliang, Zuo, Zhicai, Yu, Shumin, Wang, Ya, Ma, Xiaoping, Liu, Haifeng, Zhou, Ziyao, Hu, Yanchun, Fu, Hualin, He, Changliang, Geng, Yi, Gu, Xiaobin, Peng, Guangneng, and Zhong, Zhijun
- Subjects
CRYPTOSPORIDIUM ,TRIOSE-phosphate isomerase ,CALVES ,GLUTAMATE dehydrogenase ,GIARDIA ,AGRICULTURAL intensification - Abstract
Giardia duodenalis is a zoonotic parasitic protist and poses a threat to human and animal health. This study investigated the occurrence of G. duodenalis infection in post-weaned calves from Sichuan province, China. Faecal samples were collected from a total of 306 post-weaned calves (3–12 months old) from 10 farms, including 4 intensive feeding farms and 6 free-ranging farms. The overall infection rate of G. duodenalis was 41.2% (126/306) based on the PCR results at any of the three genetic loci: beta-giardin (bg), triose-phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes. Giardia duodenalis assemblages E (n = 115, 91.3%), A (n = 3, 2.4%), and A mixed with E (n = 8, 6.3%) were identified among the 126 positive specimens. Multilocus sequence typing of G. duodenalis revealed 34 assemblage E multilocus genotypes (MLGs), 1 assemblage A MLG and 7 mixed assemblage (A and E) MLGs. The eBURST data showed a high degree of genetic diversity within assemblage E MLGs. The phylogenetic tree revealed that MLG E3 was the primary MLG subtype in Sichuan province and also the most widely distributed in China. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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