1. Complete genomic sequence and comparative analysis of rice stripe virus from Sichuan Province, China.
- Author
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Wu, Gentu, Luo, Hao, Zheng, Guixian, Liu, Xiaoqian, and Qing, Ling
- Subjects
COMPARATIVE genomics ,RNA polymerases ,RNA replicase ,SEQUENCE analysis ,RICE ,VIRUS diseases ,NUCLEOTIDE sequence - Abstract
Rice stripe virus (RSV) causes viral disease and yield losses in rice. Leaves of rice plants showing yellowing, stripes and curling were collected in Sichuan Province, China and tested for rice strip virus (RSV) by RT-PCR using specific primers. Nine of the nineteen symptomatic samples were positive for RSV. The complete sequence of RSV RNA1 to RNA4 was determined by assembling cDNA fragments obtained from isolate Huid04 in Huidong County, Sichuan Province (GenBank accession Nos. KX611337 to KX611340). Phylogenetic analyses of RNA-dependent RNA polymerase sequences showed a close relationship of RSV isolate Hui0d4 and isolates DaL08 and KunM08 from Yunnan Province, China. This is the first complete genomic sequence of an RSV isolate from Sichuan Province, China. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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