1. DNA sequencing using a four-color confocal fluorescence capillary array scanner.
- Author
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Kheterpal I, Scherer JR, Clark SM, Radhakrishnan A, Ju J, Ginther CL, Sensabaugh GF, and Mathies RA
- Subjects
- Base Sequence, Electrophoresis, Capillary methods, Humans, Molecular Sequence Data, Sequence Analysis, DNA methods, Sierra Leone, DNA, Mitochondrial chemistry, Electrophoresis, Capillary instrumentation, Sequence Analysis, DNA instrumentation
- Abstract
The design, construction and operation of a four-color capillary array electrophoresis scanner are presented. The use of sensitive energy transfer primers facilitates four-color detection of the DNA sequencing fragments following excitation at a single laser wavelength (488 nm). This scanner collects fluorescence data from up to 25 capillaries in parallel. The resulting four-color image files are automatically reduced to four-color line plots, and a base-calling program (Sax) is used to call the sequence. The performance of this system for DNA sequencing is demonstrated by examining twelve different motifs of the hypervariable region I of human mitochondrial (mt) DNA obtained from a Sierra Leone population.
- Published
- 1996
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