1. Clonal relationship among Vibrio cholerae O1 El Tor strains isolated in Somalia.
- Author
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Scrascia M, Pugliese N, Maimone F, Mohamud KA, Grimont PA, Materu SF, and Pazzani C
- Subjects
- Anti-Bacterial Agents pharmacology, Bacterial Typing Techniques, Cluster Analysis, Conjugation, Genetic, DNA Fingerprinting, DNA, Bacterial chemistry, DNA, Bacterial genetics, Disease Outbreaks, Drug Resistance, Bacterial, Genotype, Humans, Microbial Sensitivity Tests, Molecular Sequence Data, Plasmids, Random Amplified Polymorphic DNA Technique, Ribotyping, Sequence Analysis, DNA, Somalia epidemiology, Vibrio cholerae O1 genetics, Cholera epidemiology, Cholera microbiology, Vibrio cholerae O1 classification, Vibrio cholerae O1 isolation & purification
- Abstract
One hundred and three Vibrio cholerae O1 strains, selected to represent the cholera outbreaks which occurred in Somalia in 1998-1999, were characterized by random amplified polymorphic DNA patterns, ribotyping, and antimicrobial susceptibility. All strains showed a unique amplified DNA pattern and 2 closely related ribotypes (B5a and B8a), among which B5a was the more frequently identified. Ninety-one strains were resistant to ampicillin, chloramphenicol, spectinomycin, streptomycin, sulfamethoxazole, and trimethoprim, conferred, except for spectinomycin, by a conjugative plasmid IncC. These findings indicated that the group of strains active in Somalia in the late 1990s had a clonal origin.
- Published
- 2009
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