1. Circulating recombinant form CRF02_AG in South America.
- Author
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Carrion G, Hierholzer J, Montano S, Alava A, Perez J, Guevara A, Laguna-Torres V, Mosquera C, Russell K, Chauca G, Kochel T, Birx DL, Sanchez JL, and Carr JK
- Subjects
- Adult, DNA, Viral blood, Ecuador epidemiology, Female, HIV Infections virology, HIV-1 genetics, Heteroduplex Analysis, Humans, Male, Molecular Sequence Data, Population Surveillance, Sequence Analysis, DNA, South America epidemiology, HIV Infections epidemiology, HIV-1 classification, Recombination, Genetic
- Abstract
With the objective of monitoring the distribution of HIV-1 subtypes and circulating recombinant forms (CRFs)in South America, population-based surveillance studies were performed in seven countries. Peripheral blood mononuclear cell, filter paper, fresh blood, and cocultivation samples were collected from HIV-positive patients from Colombia, Ecuador, Peru, Bolivia, Chile, Argentina, and Uruguay, during a 7-year period(1995-2001). DNA was prepared and HIV envelope subtypes were determined by heteroduplex mobility as-say and DNA sequencing from 1289 HIV-positive samples. While subtypes B and F were the most commonly observed subtypes, two CRF02_AG strains were detected, in Ecuador. This is the first report of the existence of this CRF in South America.
- Published
- 2003
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